Certificate of Laboratory Analysis
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Non-Viable Spore Trap Analysis Granville County Schools
Project #:
17-1929
Dr. Stan Winborne
Project Location:
Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
Table 1: Non-Viable Air Samples Date Collected:
10/4/17 1
10/4/17 2
10/4/17 3
10/4/17 4
10/4/17 5
CR116
Hall at CR116
CR106
CR104
Hall outside CR104/106
67 27 13 13 27 -
133 13 40 213 40 40 27 13 13 13 -
67 13 27 13 27 -
93 13 27 573 27 133 13 147 40 -
93 53 613 27 107 120 13 53 -
3
147
547
147
1067
1080
Particulate Level
low
low-moderate
low-moderate
moderate
moderate
10/5/17
10/5/17
10/5/17
10/5/17
Spore Identification Cladosporium Ascospores Basidiospores2 Smuts, Periconia , Myxomycetes4 Penicillium/Aspergillus Group1 Hyphal Elements3 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust5 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys
Total Spores/m
Date Analyzed: 10/5/17 Analyzed by: Cathy A. Richmond, B.S.
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis Granville County Schools
Project #:
17-1929
Dr. Stan Winborne
Project Location:
Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
Table 1: Non-Viable Air Samples Date Collected:
10/4/17 6
Spore Identification Cladosporium Ascospores Basidiospores2 Smuts, Periconia , Myxomycetes4 Penicillium/Aspergillus Group1 Hyphal Elements3 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust5 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys
Total Spores/m
3
Particulate Level
Outdoor Air 1147 160 2347 1013 107 53 160 80 5067 low-moderate
Date Analyzed: 10/5/17 Analyzed by: Cathy A. Richmond, B.S.
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
1 Volume (L): Sample Number: CR116 Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 5 67 Ascospores Basidiospores 2 27 Smuts, Periconia , Myxomycetes 1 13 Penicillium/Aspergillus Group 1 13 Hyphal Elements 2 27 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 11 147 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low _ Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 45% 18% 9% 9% 18% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
2 Volume (L): Sample Number: Hall at CR116 Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 10 133 Ascospores 1 13 Basidiospores 3 40 Smuts, Periconia , Myxomycetes 16 213 Penicillium/Aspergillus Group 3 40 Hyphal Elements 3 40 Alternaria Curvularia 2 27 Epicoccum 1 13 Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces 1 13 Rust Drechslera/Bipolaris Tetraploa 1 13 Chaetomium Stachybotrys Total Spores 41 547 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low-moderate _ Units Percentage spores/m3 24% 3 spores/m 2% spores/m3 7% spores/m3 39% spores/m3 7% 7% spores/m3 spores/m3 spores/m3 5% spores/m3 2% spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 3 spores/m spores/m3 spores/m3 2% spores/m3 spores/m3 spores/m3 2% spores/m3 spores/m3 spores/m3 spores/m3
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
3 Volume (L): Sample Number: CR106 Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 5 67 Ascospores 1 13 Basidiospores Smuts, Periconia , Myxomycetes 2 27 Penicillium/Aspergillus Group 1 13 Hyphal Elements 2 27 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 11 147 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low-moderate _ Units Percentage spores/m3 45% 3 spores/m 9% spores/m3 spores/m3 18% spores/m3 9% 18% spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 3 spores/m spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
4 Volume (L): Sample Number: CR104 Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 7 93 Ascospores 1 13 Basidiospores 2 27 Smuts, Periconia , Myxomycetes 43 573 Penicillium/Aspergillus Group 2 27 Hyphal Elements 10 133 Alternaria 1 13 Curvularia 11 147 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces 3 40 Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 80 1067 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate _ Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 9% 1% 3% 54% 3% 13% 1% 14% 4% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
5 Volume (L): Sample Number: Hall outside CR104/106 Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 7 93 Ascospores Basidiospores 4 53 Smuts, Periconia , Myxomycetes 46 613 Penicillium/Aspergillus Group 2 27 Hyphal Elements 8 107 Alternaria Curvularia 9 120 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium 1 13 Torula Pithomyces 4 53 Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 81 1080 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate _ Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 9% 5% 57% 2% 10% 11% 1% 5% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-1929
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Credle Elementary
P.O. Box 927
223 College Street
Oxford, NC 27565
Oxford, NC Project Type:
CLR
PO/Claim #:
6 Volume (L): Sample Number: Outdoor Air Percentage of Slide Read: Sample Location: 10/4/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 10/5/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 43 1147 Ascospores 6 160 Basidiospores 88 2347 Smuts, Periconia , Myxomycetes 38 1013 Penicillium/Aspergillus Group 4 107 Hyphal Elements 2 53 Alternaria Curvularia 6 160 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces 3 80 Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 190 5067 Analyzed by: Cathy A. Richmond, B.S.
75 50.0% 26.67 low-moderate _ Units Percentage spores/m3 23% 3 spores/m 3% spores/m3 46% spores/m3 20% spores/m3 2% 1% spores/m3 spores/m3 spores/m3 3% spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 3 spores/m spores/m3 spores/m3 2% spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Project #:
17-1929
Report Information: DETECTION LIMITS (DL) for samples are the minimum number of spores or colonies forming units that can be satisfactorily identified for each sample type. SPORE TRAP SAMPLES: Calculations based on volume of air sampled & percentage of slide counted, i.e. DL = 1000 L / 75 L if 100% of the slide is counted. CODE 11: Fungal content and/or particulate level on slide too heavy to identify and enumerate fungal content.
Footnotes: 1. Penicillium/Aspergillus group spores are characterized by their small size, round to ovoid shape, being unicellular and usually colorless to lightly pigmented. There are numerous genera of fungi whose spore morphology is similar to that of the Penicillium/Aspergillus type. Several common examples would be Acremonium, Paecilomyces, and Trichoderma . Although the majority of spores placed in this group are Penicillium , Aspergillus , or a combination of both, these are not the only two possibilities. 2. Basidiospores are primarily transported indoors from outdoor sources and rarely grow indoors. A high basidiospore count indoors can be indicative of a wood decay problem or wet soil, and should be verified if and an outdoor source of the spores is not present. 3. Hyphae are the tubular filaments of fungi. Hyphae can fragment and become airborne much like spores and are potentially allergenic. 4. The Smut, Periconia, Myxomycete group is a group composed of three different types of organisms whose spores have similar morphologies. Smuts are plant pathogens, Periconia is a relatively uncommon mold indoors, and Myxomycetes are not fungi, but slime molds. Although these organisms do not typically proliferate indoors, their spores are potentially allergenic. 5. Rusts are plant pathogens. These fungi do not typically grow indoors unless an infected plant is present. Rust spores are potentially allergenic.
Direct Microscopic Exam Reporting: We use a 400x-600x magnification microscope. Reporting Quantification Levels are as follows: Reporting Level Occasional Few
Quantitative Description 1-10 per square inch 11-100 per square inch
Moderate
101-1000 per square inch
Numerous
More than 1,000 per square inch
Submitted By Analyst: Cathy A. Richmond, BS
