Certificate of Laboratory Analysis
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Non-Viable Spore Trap Analysis Granville County Schools
Project #:
17-2104
Dr. Stan Winborne
Project Location:
Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
Table 1: Non-Viable Air Samples Date Collected:
Spore Identification Cladosporium Ascospores Basidiospores2 Smuts, Periconia , Myxomycetes4 Penicillium/Aspergillus Group1 Hyphal Elements3 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust5 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys
Total Spores/m
3
Particulate Level
11/14/17 1
11/14/17 2
11/14/17 3
11/14/17 4
11/14/17 5
Cafeteria front
Cafeteria rear
Music room
Classroom 111
Classroom 3
693 1213 1347 53 67 40 -
267 1653 1240 107 67 27 13 13 -
173 80 160 893 80 120 67 27 13 -
200 120 400 80 93 53 13 13 -
213 67 227 1120 80 307 147 13 40 13 27 13 -
3413
3387
1613
973
2267
low
low-moderate
moderate
moderate
moderate-heavy
11/15/17
11/15/17
11/15/17
11/15/17
Date Analyzed: 11/15/17 Analyzed by: Cathy A. Richmond, B.S.
Certificate of Laboratory Analysis
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Non-Viable Spore Trap Analysis Granville County Schools
Project #:
17-2104
Dr. Stan Winborne
Project Location:
Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
Table 1: Non-Viable Air Samples Date Collected:
Spore Identification Cladosporium Ascospores Basidiospores2 Smuts, Periconia , Myxomycetes4 Penicillium/Aspergillus Group1 Hyphal Elements3 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust5 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys
Total Spores/m
3
Particulate Level
11/14/17 6
11/14/17 7
11/14/17 8
11/14/17 9
11/14/17 10
Classroom 6
Classroom 10
Media Center
Classroom 303
Outdoor air
93 53 53 27 53 13 13 -
133 53 80 93 27 80 13 13 27 -
93 27 107 40 13 -
147 13 40 827 53 80 53 40 13 13 13 -
1387 2720 2987 1280 373 53 160 -
307
520
280
1293
8960
low-moderate
low-moderate
low
moderate
low-moderate
11/15/17
11/15/17
11/15/17
11/15/17
Date Analyzed: 11/15/17 Analyzed by: Cathy A. Richmond, B.S.
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis Granville County Schools
Project #:
17-2104
Dr. Stan Winborne
Project Location:
Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
1 Volume (L): Sample Number: Cafeteria front Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 52 693 Ascospores 91 1213 Basidiospores 101 1347 Smuts, Periconia , Myxomycetes 4 53 Penicillium/Aspergillus Group 5 67 Hyphal Elements 3 40 Alternaria Curvularia Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 256 3413 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 20% 36% 39% 2% 2% 1% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
2 Volume (L): Sample Number: Cafeteria rear Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 20 267 Ascospores 124 1653 Basidiospores 93 1240 Smuts, Periconia , Myxomycetes 8 107 Penicillium/Aspergillus Group 5 67 Hyphal Elements 2 27 Alternaria Curvularia 1 13 Epicoccum Cercospora 1 13 Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 254 3387 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low-moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 8% 49% 37% 3% 2% 1% 0% 0% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
3 Volume (L): Sample Number: Music room Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 13 173 Ascospores 6 80 Basidiospores 12 160 Smuts, Periconia , Myxomycetes 67 893 Penicillium/Aspergillus Group 6 80 Hyphal Elements 9 120 Alternaria Curvularia 5 67 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified 2 27 Ulocladium Torula Pithomyces Rust 1 13 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 121 1613 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 11% 5% 10% 55% 5% 7% 4% 2% 1% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
4 Volume (L): Sample Number: Classroom 111 Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 15 200 Ascospores Basidiospores 9 120 Smuts, Periconia , Myxomycetes 30 400 Penicillium/Aspergillus Group 6 80 Hyphal Elements 7 93 Alternaria Curvularia 4 53 Epicoccum Cercospora Arthrinium 1 13 Clear Brown Colorless Trichocladium Unidentified 1 13 Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 73 973 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 21% 12% 41% 8% 10% 5% 1% 1% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
5 Volume (L): Sample Number: Classroom 3 Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 16 213 Ascospores 5 67 Basidiospores 17 227 Smuts, Periconia , Myxomycetes 84 1120 Penicillium/Aspergillus Group 6 80 Hyphal Elements 23 307 Alternaria Curvularia 11 147 Epicoccum Cercospora Arthrinium 1 13 Clear Brown Colorless Trichocladium Unidentified 3 40 Ulocladium Torula Pithomyces 1 13 Rust 2 27 Drechslera/Bipolaris 1 13 Tetraploa Chaetomium Stachybotrys Total Spores 170 2267 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate-heavy Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 9% 3% 10% 49% 4% 14% 6% 1% 2% 1% 1% 1% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
6 Volume (L): Sample Number: Classroom 6 Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 7 93 Ascospores Basidiospores 4 53 Smuts, Periconia , Myxomycetes 4 53 Penicillium/Aspergillus Group 2 27 Hyphal Elements 4 53 Alternaria Curvularia 1 13 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified 1 13 Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 23 307 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low-moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 30% 17% 17% 9% 17% 4% 4% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
7 Volume (L): Sample Number: Classroom 10 Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 10 133 Ascospores 4 53 Basidiospores 6 80 Smuts, Periconia , Myxomycetes 7 93 Penicillium/Aspergillus Group 2 27 Hyphal Elements 6 80 Alternaria Curvularia 1 13 Epicoccum 1 13 Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces 2 27 Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 39 520 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low-moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 26% 10% 15% 18% 5% 15% 3% 3% 5% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
8 Volume (L): Sample Number: Media Center Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 7 93 Ascospores Basidiospores 2 27 Smuts, Periconia , Myxomycetes 8 107 Penicillium/Aspergillus Group 3 40 Hyphal Elements Alternaria Curvularia 1 13 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 21 280 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 low Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 33% 10% 38% 14% 5% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
9 Volume (L): Sample Number: Classroom 303 Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 11 147 Ascospores 1 13 Basidiospores 3 40 Smuts, Periconia , Myxomycetes 62 827 Penicillium/Aspergillus Group 4 53 Hyphal Elements 6 80 Alternaria Curvularia 4 53 Epicoccum 3 40 Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified 1 13 Ulocladium Torula Pithomyces 1 13 Rust 1 13 Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 97 1293 Analyzed by: Cathy A. Richmond, B.S.
75 100.0% 13.33 moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 11% 1% 3% 64% 4% 6% 4% 3% 1% 1% 1% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Non-Viable Spore Trap Analysis 17-2104
Granville County Schools
Project #:
Dr. Stan Winborne
Project Location: Creedmoor Elementary
P.O. Box 927
305 E. Wilton
Oxford, NC 27565
Creedmoor, NC Project Type:
IAQ
PO/Claim #:
10 Volume (L): Sample Number: Outdoor air Percentage of Slide Read: Sample Location: 11/14/17 Detection Limit: Date Collected: Non-viable spore trap analysis Particulate Level: Test Requested: 11/15/17 Notes: Date Analyzed: Spore Identification Count Results Cladosporium 26 1387 Ascospores 51 2720 Basidiospores 56 2987 Smuts, Periconia , Myxomycetes 24 1280 Penicillium/Aspergillus Group 7 373 Hyphal Elements 1 53 Alternaria Curvularia 3 160 Epicoccum Cercospora Arthrinium Clear Brown Colorless Trichocladium Unidentified Ulocladium Torula Pithomyces Rust Drechslera/Bipolaris Tetraploa Chaetomium Stachybotrys Total Spores 168 8960 Analyzed by: Cathy A. Richmond, B.S.
75 25.0% 53.33 low-moderate Units spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3 spores/m3
Percentage 15% 30% 33% 14% 4% 1% 2% -
LRC Indoor Testing and Research 140 Iowa Lane, Suite 102 Cary, NC 27511 (919) 342-4936
Certificate of Laboratory Analysis Project #:
17-2104
Report Information: DETECTION LIMITS (DL) for samples are the minimum number of spores or colonies forming units that can be satisfactorily identified for each sample type. SPORE TRAP SAMPLES: Calculations based on volume of air sampled & percentage of slide counted, i.e. DL = 1000 L / 75 L if 100% of the slide is counted. CODE 11: Fungal content and/or particulate level on slide too heavy to identify and enumerate fungal content.
Footnotes: 1. Penicillium/Aspergillus group spores are characterized by their small size, round to ovoid shape, being unicellular and usually colorless to lightly pigmented. There are numerous genera of fungi whose spore morphology is similar to that of the Penicillium/Aspergillus type. Several common examples would be Acremonium, Paecilomyces, and Trichoderma . Although the majority of spores placed in this group are Penicillium , Aspergillus , or a combination of both, these are not the only two possibilities. 2. Basidiospores are primarily transported indoors from outdoor sources and rarely grow indoors. A high basidiospore count indoors can be indicative of a wood decay problem or wet soil, and should be verified if and an outdoor source of the spores is not present. 3. Hyphae are the tubular filaments of fungi. Hyphae can fragment and become airborne much like spores and are potentially allergenic. 4. The Smut, Periconia, Myxomycete group is a group composed of three different types of organisms whose spores have similar morphologies. Smuts are plant pathogens, Periconia is a relatively uncommon mold indoors, and Myxomycetes are not fungi, but slime molds. Although these organisms do not typically proliferate indoors, their spores are potentially allergenic. 5. Rusts are plant pathogens. These fungi do not typically grow indoors unless an infected plant is present. Rust spores are potentially allergenic.
Direct Microscopic Exam Reporting: We use a 400x-600x magnification microscope. Reporting Quantification Levels are as follows: Reporting Level Occasional Few
Quantitative Description 1-10 per square inch 11-100 per square inch
Moderate
101-1000 per square inch
Numerous
More than 1,000 per square inch
Submitted By Analyst: Cathy A. Richmond, BS
