Blood RNAViroid Preservation and Purification Detection Kits System

NORGEN CORP. BIOTEK

Features Input blood volumes of 3 mL Preservation of whole blood RNA for up to 12 days at room temperature RNA purity with A260/280 ratio >1.8 Isolate 6 to 25 μg of RNA from 3 mL blood No enzymatic treatment required

A Complete System for the Preservation and Isolation of Whole Blood RNA

No specialized equipment necessary B

A

Day 6 Preservation at RT

24

Average Ct Value

21 18 15 12 9 6 3 0 Day 0

Day 6

Day 12

Days of Blood RNA Preservation 18S rRNA

FOS

L-1B

Figure 1. Stability of Blood RNA in Norgen’s Blood RNA Preservative Tubes. A main component of Norgen’s Blood RNA Preservation and Purification Kits are the Blood RNA Preservative Tubes. Blood samples were collected from the same donor in triplicate on Norgen’s Blood RNA Preservative Tubes. The stability of the blood RNA in Norgen’s Blood RNA Preservative Tubes was evaluated over a period of 12 days at room temperature. At each time point, the preserved blood RNA was isolated using Norgen’s Blood RNA Purification Kit. Three different genes, FOS, IL1B and 18S rRNA, were used to evaluate the stability of blood RNA over a period of 12 days at room temperature. Figure 1A shows the efficiency of Norgen’s Blood RNA Preservative for minimizing the induction of gene expression over a period of 12 days at Room temperature. Each of the 3 genes tested showed no changes in gene expression over the 12 day preservation period. Figure 1B shows the quality of the Blood RNA preserved for 6 days at room temperature. As it can be seen, Norgen’s Preserved Blood RNA Purification method allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol.

North American Toll-Free: 1-866-667-4362 Phone: 905-227-8848 Fax: 905-227-1061 [email protected] www.norgenbiotek.com

ISO 13485:2003, ISO 9001:2008, ISO 15189:2007 Registered

Blood RNAViroid Preservation and Purification Detection Kits System A

35

A

30 25

25

Ct Value

20 15 10 5 0

Lot 1

Lot 2

Lot 3

B

2 1.5

25

1 0.5 Lot 1

Lot 2

Ct Value

A260/280

15

Day 1

Day 2

Days of Blood RNA Preservation at Room Temperature

Lot 1

3 2.5

0

20

10

Day 0 Day 6 Day 12

B

Consistency and Repeatability of 3 different Lots from Norgen’s Blood RNA Preservative evaluated by following the preservation of the 18S rRNA

Lot 3

Lot 2

Day 3

Lot 3

Consistency and Repeatability of 3 different Lots from Norgen’s Blood RNA Preservative evaluated by following the preservation of the IL1B RNA Transcript

20 15

Day 0 Day 6 Day 12 10

Day 1

Day 2

Days of Blood RNA Preservation at Room Temperature

Lot 1

C 25

Ct Value

Figure 2. Qualitative and Quantitative Analysis of Blood RNA Preserved and Isolated using Norgen’s Blood RNA Preservation and Purification System. Preserved blood RNA was processed using three different lots of Norgen’s Blood RNA Preservation and Purification System and were analyzed qualitatively and quantitatively. Blood was collected from the same donor on Norgen’s Blood RNA Preservative Tubes. Blood RNA preservation was evaluated for the three different lots over a period of 12 days at room temperature. Preserved blood RNA was isolated from each sample at each time point using Norgen’s Blood RNA Preservation System. Figure A is showing the RNA yield from blood isolated and preserved for 12 days at room temperature.  Figure B is showing the high quality of the RNA isolated from blood preserved for 12 days at room temperature.

Lot 2

Day 3

Lot 3

Consistency and Repeatability of 3 different Lots from Norgen’s Blood RNA Preservative evaluated by following the preservation of the FOS RNA Transcript

20 15 10

Day 1

Day 2

Days of Blood RNA Preservation at Room Temperature

Lot 1

Lot 2

Day 3

Lot 3

Figure 3. Consistency and Repeatability of Norgen’s Blood RNA Preservation and Purification System. Three different lots of Norgen’s Blood RNA Preservation and Purification System were tested for consistency and repeatability. Blood was collected from the same donor on Norgen’s Blood RNA Preservative Tubes. Blood RNA preservation was evaluated for the three different lots over a period of 12 days at room temperature. Preserved blood RNA was isolated from each sample at each time point using Norgen’s Blood RNA Purification Kit. The preservation of blood RNA was analyzed quantitatively using RT-qPCR amplification of FOS-gene, IL1B gene ad 18S rRNA. Figures 3A, 3B and 3C shows the efficiency of the three different lots of Norgen’s Blood RNA Preservative Tubes on minimizing the gene induction of the three tested genes (18S rRNA, IL1B and FOS, respectively) with no significant difference between the three different lots.

Cat. #

Description

Quantity

52620 / 52720

Blood RNA Preservation and Purification System

48 Preps / 96 Preps

Cat. #

Related Products

52600 / 52700

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North American Toll-Free: 1-866-667-4362 Phone: 905-227-8848 Fax: 905-227-1061 [email protected] www.norgenbiotek.com

ISO 13485:2003, ISO 9001:2008, ISO 15189:2007 Registered