Q3/9B/04-13 Reg. No

St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001 AOBI301 - BIOINSTRUMENTATION

Time : 3 hrs

Max Marks :75 SECTION – A (25X1=25) Answer ALL Questions

I. Choose the correct answer : 1. Clinical centrifuge is also called as --------- centrifuge. a) High speed b) Desk top c) Ultra d) None 2. The most predominant buffer in body is a) Bicarbonate buffer b) Phosphate c) Protein 3. The pH of blood is a) 5.4 b) 6.8

c) 7.4

d) Both a &b

d) 8.2

4. The separation technique that depends only upon molecular size is a) Gel filtration chromatography b) TLC c) GLC d) Paper chromatography 5. Electrophoresis with a stabilizing media is called -----------. a) Free electrophoresis b) Zone electrophoresis c) Both d) None 6. One Svedberg unit is equal to -----------. a) 1 x 10-10 sec b) 1 x 10-16 sec c) 1 x 10-13 sec 7. Buffers have the ability to a) Change the pH b) Resist change in pH 8. GLC is a type of ----------------- chromatography a) Adsorption b) Partition c) Adhesion

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d) 1 x 10-12 sec

c) Both

d) None

d) None

Q3/9B/04-13 9. The pH scale ranges from _____________. a) 0-7 b) 0-14 c) 1-14

d) 1-7

10. Amino acids are detected by the reagent _____________. a) Ninhydrin b) Sulphuric acid c) Potasiumpermanganate d) None of them II. Say true or false : 11. Alkaline solutions will have pH value less than 7. 12. The sharpness of separation is better in TLC than in paper chromatography. 13. Separation in centrifugation is based on molecular weight. 14. The plate containing stationary phase must be activated before applying sample. 15. The moving boundary Electrophoresis was first developed by Tiselius. III. Answer in one or two sentences : 16. What is buffer capacity? 17. Why is Rf value of any solute is below 1? 18. What is isoelectric point? 19. Define molarity. 20. What is a reference electrode? 21. What is bed volume? 22. How 1N solution of NaOH is prepared?

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Q3/9B/04-13 23. What is partition chromatography? 24. Write the name of the binder used in TLC. 25. What is the pH of the urine? SECTION – B (4X5=20) Answer any FOUR Questions 26. Describe the principle and applications of TLC. 27. Give an account on paper electrophoresis. 28. What are the factors affecting Electrophoretic mobility? 29. Describe gel permeation chromatography briefly. 30. Explain isopycnic centrifugation technique. SECTION – C (3X10=30) Answer any THREE Questions 31. Describe the construction of GLASS electrode and its merits. 32. Explain analytical centrifugation in detail. 33. Write in detail about the following buffers. a) Haemoglobin buffer b) Bicarbonate buffer 34. Give an account on SDS-PAGE electrophoresis. 35. Explain HPLC in detail?

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