IGENITY® Phone: 402-435-0665 Fax: 402-435-0664 e-mail:[email protected]

Boyer Limousin 15464 E State Highway 8 Liberty, NE 68381-8012

Date 2/27/18 Customer ID BOY010 Additional Tests

Animal ID

Sample Barcode #

M/F

Breed

BVD PI

Coat Color

9796D

81512121756

Yes

3152D

81512121790

Yes

8913D

81512121789

Yes

66D

81512121787

Yes

687D

81512121766

Yes

346D

81512121765

Yes

1068D

81512121757

Yes

253D

81512121764

No

6643D

81512121780

Yes

255D

81512121763

Yes

64D

81512121762

Yes

182D

81512121760

No

197D

81512121782

No

521E

9517012667

Yes

14E

9517012668

Yes

10E

9517012669

Yes

01E

9517012662

Yes

Horned/ Polled

Myostatin

Red Charlie

Results Key BVD: (Bovine Viral Diarrhea)

Negative = no BVDV detected Positive = BVDV detected Inconclusive = weakly positive for BVDV but requires confirmatory testing Please submit a whole blood sample collected at least three weeks after the initial sample was collected.

Coat Color (homozygous black):

Yes = homozygous black (ED ED) No = not homozygous black (genotype available upon request)

Horned/Polled:

PP = homozygous polled HP = heterozygous horned/polled HH = homozygous horned

Myostatin:

0 = none of the nine possible variants are present 1, Variant = one copy of the listed variant is present 2, Variant = two copies of the listed variant are present Possible variants are C313Y, nt419, E226X, nt821, E291X, Q204X, D182N, F94L, and S105C. Free = Red Charlie Free Carrier = Red Charlie Carrier Affected = Red Charlie Affected

Red Charlie:

98749

We invite you to learn more about applying your test results, and viewing your personal online results database, by visting www.igenity.com. Order more collection kits, review research and gain management advice at this website. This report describes results of our analysis of the sample or samples of biological materials provided by you and described herein. Neogen warrants only that it has applied its best commercial efforts to the determination of the presence and identification of specific alleles of bovine genes in DNA from the sample(s) provided herein to Neogen from you. Neogen provides no other warranty of any kind, whether express or implied, (including, but not limited to, all warranties of merchantability, fitness for a particular purpose, title, noninfringement, or that any other allele is or is not present in any sample or any animal). Results of analysis are predicated on the assumption that each sample was obtained from a single cattle beast. Results of analysis are reported herein in association with the sample designations provided by you. Neogen assumes no responsibility for correctly identifying a particular animal as the source of any sample. Neogen assumes no legal liability or responsibility for the accuracy, completeness, reliability or usefulness of any information disclosed in this report, nor does Neogen represent that its use would not infringe privately owned rights. In no event shall Neogen or its agents or officers be liable for any damages whatsoever (including without limitation, damages for loss of profits or business interruption, or any indirect, special, punitive, consequential or incidental damages) arising out of the use of the results and information provided herein, even if Neogen has been advised of the possibility of such damages.

1

IGENITY® Phone: 402-435-0665 Fax: 402-435-0664 e-mail:[email protected]

Boyer Limousin 15464 E State Highway 8 Liberty, NE 68381-8012

Date 2/27/18 Customer ID BOY010 Additional Tests

Animal ID

Sample Barcode #

M/F

Breed

BVD PI

Coat Color

04E

9517012269

Yes

8E

9517012666

Yes

02E

9517012645

Yes

3709D

81512121791

Yes

236D

12313021313

Yes

25D

81512121781

Yes

3184D

81512121758

Yes

Horned/ Polled

Myostatin

Red Charlie

Results Key BVD: (Bovine Viral Diarrhea)

Negative = no BVDV detected Positive = BVDV detected Inconclusive = weakly positive for BVDV but requires confirmatory testing Please submit a whole blood sample collected at least three weeks after the initial sample was collected.

Coat Color (homozygous black):

Yes = homozygous black (ED ED) No = not homozygous black (genotype available upon request)

Horned/Polled:

PP = homozygous polled HP = heterozygous horned/polled HH = homozygous horned

Myostatin:

0 = none of the nine possible variants are present 1, Variant = one copy of the listed variant is present 2, Variant = two copies of the listed variant are present Possible variants are C313Y, nt419, E226X, nt821, E291X, Q204X, D182N, F94L, and S105C. Free = Red Charlie Free Carrier = Red Charlie Carrier Affected = Red Charlie Affected

Red Charlie:

98749

We invite you to learn more about applying your test results, and viewing your personal online results database, by visting www.igenity.com. Order more collection kits, review research and gain management advice at this website. This report describes results of our analysis of the sample or samples of biological materials provided by you and described herein. Neogen warrants only that it has applied its best commercial efforts to the determination of the presence and identification of specific alleles of bovine genes in DNA from the sample(s) provided herein to Neogen from you. Neogen provides no other warranty of any kind, whether express or implied, (including, but not limited to, all warranties of merchantability, fitness for a particular purpose, title, noninfringement, or that any other allele is or is not present in any sample or any animal). Results of analysis are predicated on the assumption that each sample was obtained from a single cattle beast. Results of analysis are reported herein in association with the sample designations provided by you. Neogen assumes no responsibility for correctly identifying a particular animal as the source of any sample. Neogen assumes no legal liability or responsibility for the accuracy, completeness, reliability or usefulness of any information disclosed in this report, nor does Neogen represent that its use would not infringe privately owned rights. In no event shall Neogen or its agents or officers be liable for any damages whatsoever (including without limitation, damages for loss of profits or business interruption, or any indirect, special, punitive, consequential or incidental damages) arising out of the use of the results and information provided herein, even if Neogen has been advised of the possibility of such damages.

2

Boyer Limousin_NE_98749_ADD ON.pdf

Customer ID BOY010. Additional Tests. Animal ID Sample Barcode # M/F Breed BVD PI Coat Color Horned/ Polled Myostatin Red Charlie. 9796D 81512121756 Yes. 3152D 81512121790 Yes. 8913D 81512121789 Yes. 66D 81512121787 Yes. 687D 81512121766 Yes. 346D 81512121765 Yes. 1068D 81512121757 Yes.

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