Dr. Giuseppe Pani, PhD Nutritional Biochemistry and Space Biology Lab. Dipartimento di Scienze Farmacologiche e Biomolecolari (DiSFEB) Sezione di Biochimica, Biofisica, Fisiologia ed Immunopatologia Facoltà di Scienze del Farmaco Università degli Studi di Milano Via D. Trentacoste 2 , 20134 Milano Phones: +39 02 50315797 Mobile: +39 349 6087543; e-mail: [email protected] Skype: Giuseppe Pani https://www.researchgate.net/profile/Giuseppe_Pani He begin to work in space biology in 2004 during the training at Sassari University to perform a master thesis on ‘’A study on human monocytes motility and cytoskeleton at real low gravity on board International Space Station (ISS)’’. He actively participated in organizing and analysing the experiment performed aboard the ISS by the cosmonaut Pavel Vinogradov. He was also involved in testing the MIA space flight hardware (Experimental Units designed to expose adherent cell to real microgravity) to obtain the ESA safety certificate for space flight. During the post-degree period, he continued his research on cytoskeleton recovery in immune system adherent cells, on osteoblast differentiation and bone matrix deposition in simulated microgravity, as well as, he was involved in space flight hardware preparation of other 2 experiments performed aboard the ISS : LEUKIN and ROALD. In June 2013, he obtained the PhD in Applied Biology. During the PhD studies at Ghent University and at the Belgian Nuclear Research Centre he continued to work in space biology performing a thesis on “Effect of space condition on neuronal plasticity and connectivity’’, based on space biology, neuroscience, bio-imaging and radioprotection. Over the PhD, he performed several experiment in simulated microgravity as well as space radiations and he contributed to develop the combined space condition facility. During this period, he was involved in other Space-related experiments on mice, plants and bacteria. Actually, he is post-doc in Space Laboratories, Department of Pharmacological and Bimolecular Sciences, University of Milan, in osteology and space biology under the supervision of Dr. Rizzo. He is involved in analysing the effects of simulated and real microgravity on osteoblast differentiation and bone matrix deposition, as well as, in testing the space flight hardware STROMA produced by Kayser Italia designed for experiment aboard the ISS. Over the PhD and the post-doc period, he got specialized in bio-imaging and he developed few algorithm bio-image analysis, in particular MorphoNeuroNet used for high content analysis of in vitro neuronal network, as well as, CellPositivity for expression or co-expression of protein in single cells, cytosolic protein distribution and cytoskeleton analysis and apoptosis. He was also involved in analysing plants and bacteria. At the moment, he is developing tools for protein quantification and distribution in osteoblast, as well as, for analysing the bone matrix deposition. Finally, he has several published articles on space biology or microscopy and he obtained awards as the 1st place at the “International Antibody Image Contest 2013”, the 9th place at the “2008 European Sigma ANTIBODY Photo Award”, poster award and images selected for international brochures and books.

Journal Publications Roel Quintens, Tine Verreet, An Janssen, Mieke Neefs, Liselotte Leysen, Arlette Michaux, Mieke Verslegers, Nada Samari, Giuseppe Pani, Joris Verheyde, Sarah Baatout, M. Abderrafi Benotmane: Identification of novel radiationinduced p53-dependent transcripts extensively regulated during mouse brain development. Biology Open. 02/2015; Angela Maria Rizzo, Paola Antonia Corsetto, Francesca Farina, Gigliola Montorfano, Giuseppe Pani, Cristina Battaglia, Giulio Sancini, Paola Palestini: Repeated Intratracheal Instillation of PM10 Induces Lipid Reshaping in Lung Parenchyma and in Extra-Pulmonary Tissues.. PLoS ONE 09/2014; 9(9):e106855.

Giuseppe Pani, Winnok H De Vos, Nada Samari, Louis de Saint-Georges, Sarah Baatout, Patrick Van Oostveldt, Mohammed Abderrafi Benotmane: MorphoNeuroNet: An automated method for dense neurite network analysis.. Cytometry Part A 11/2013; Giuseppe Pani, Nada Samari, Roel Quintens, Louis de Saint-Georges, Mariantonia Meloni, Sarah Baatout, Patrick Van Oostveldt, Mohammed Abderrafi Benotmane: Morphological and Physiological Changes in Mature In Vitro Neuronal Networks towards Exposure to Short-, Middle- or Long-Term Simulated Microgravity.. PLoS ONE 09/2013; 8(9):e73857. Nada Samari, Louis De Saint-Georges, Giuseppe Pani, Sarah Baatout, Luc Leyns, Mohammed Abderrafi Benotmane: Non-conventional apoptotic response to ionising radiation mediated by N-methyl D-aspartate receptors in immature neuronal cells.. International Journal of Molecular Medicine 01/2013; 31(3). Maria Antonia Meloni, Grazia Galleri, Giuseppe Pani, Angela Saba, Proto Pippia, Marianne Cogoli-Greuter: Space flight affects motility and cytoskeletal structures in human monocyte cell line J-111.. Cytoskeleton 02/2011; 68(2):12537. P. Pippia, G. Galleri, M.A. Meloni, A. Saba, G. Pani, M. Cogoli-Greuter, A. Cogoli: Effects of microgravity and cosmic radiations on human T lymphocytes. Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale. 01/2011; 82(1). Alessio Fortunati, Paola Tassone, Angela Saba, Giuseppe Pani, Maria Antonietta Meloni, Proto Pippia, Fernando Migliaccio: Alterations in the expression of genes for tubulin and stress in two Arabidopsis auxin mutants during simulated microgravity. Journal of Biological Research 01/2011; 84(1):67. Maria Antonia Meloni, Giuseppe Pani, Rafi Benotmane, Felice Mastroleo, Khalil Aboul-El-Ardat, Ann Janssen, Liselotte Leysen, Filip Vanhavere, Natalie Leys, Grazia Galleri, Proto Pippia, Sarah Baatout: Effects of ISS equivalent ionizing radiation dose on Human T-lymphocytes. 01/2010; A. Saba, G. Pani, G. Galleri, M.A. Meloni, P. Pippia: "In vivo" physiological experiments in simulated microgravity conditions on rat bon marrow cells mineralization. Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale. 01/2009; 82(1).