[LA 0412]

Sub. Code: 1203 M.Sc BIOCHEMISTRY DEGREE EXAMINATION Candidates admitted from 2008-2009 batch PAPER III – MOLECULAR BIOLOGY, CLINICAL BIOCHEMISTRY, FUNCTION TESTS, ENDOCRINILOGY, IMMUNOLOGY, RECENT ADVANCES IN BIOCHEMISTRY Q.P. Code : 281203 Time : Three hours Maximum :100marks Answer All questions. I. Elaborate on : Pages Time Marks (Max.) (Max.) (Max.) 1.

What is Blot transfer technique? Briefly explain the various types of blot transfer techniques and their applications.

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What are Proto-oncogenes? Explain the normal mechanism of action of proto-oncogenes and how they are activated to oncogenes.

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Anion gap.

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Monoclonal antibodies and their clinical significance.

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5. Explain mechanism of hormone action through cytosolic                 or nuclear receptors. 4

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What is a buffer? Explain how the various buffers help to maintain acid base status. II. Write notes on : 1. What is genetic code? What are the features of the genetic code? 2.

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Transport of iron in plasma and its storage.

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7. What are the biochemical investigations to assess the                  tabular function of kidneys?

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Alkaline phosphatase and its clinical importance.

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9. Enumerate and describe briefly the pre-analytical                 errors in clinical laboratory

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10. Laboratory Information System. *******

[LB 1012]

OCTOBER 2012 Sub. Code: 1203 M.Sc BIOCHEMISTRY DEGREE EXAMINATION (Candidates admitted from 2008-2009 batch) PAPER III – MOLECULAR BIOLOGY, CLINICAL BIOCHEMISTRY, FUNCTION TESTS, ENDOCRINILOGY, IMMUNOLOGY, RECENT ADVANCES IN BIOCHEMISTRY Q.P. Code : 281203 Time : 3 hours Maximum : 100 marks (180 Min) Answer ALL questions in the same order. I. Elaborate on : Pages Time Marks (Max.)(Max.)(Max.) 1. Describe in detail Recombinant DNA technology and its 17 40 20 application in medicine. Add a note on molecular scissors. 2. How is acid- base balance regulated in the body?

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II. Write Notes on : 1. Investigations to be performed in a case of Nephrotic syndrome. 4 2. Estimation of blood glucose with a note on sample required,   interferences and reference range. 4 3. Describe the various post-transcriptional modifications. 4 4. Mention the normal levels of serum electrolytes. Detail about the sodium homeostasis in our body. 4 5. Evaluation of thyroid function in a case of goiter. 4 6. Hyperuricemia. 4 7. Describe in detail western blotting technique and its applications. 4 8. Describe about mitochondrial DNA. 4 9. Describe the various tumour markers and their applications. 4 10. Estimation of amylase in the laboratory with a note on sample required and its clinical applications. 4 *******

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[LC 0413]

APRIL 2013 Sub. Code: 1203 M.Sc BIOCHEMISTRY DEGREE EXAMINATION Candidates admitted from 2008-2009 batch PAPER III – MOLECULAR BIOLOGY, CLINICAL BIOCHEMISTRY, FUNCTION TESTS, ENDOCRINILOGY, IMMUNOLOGY, RECENT ADVANCES IN BIOCHEMISTRY Q.P. Code : 281203 Time : 3 hours Maximum : 100 marks I. Elaborate on:

(2x20=40)

1. How is uric acid produced in the body? Add a note on disorders associated with it. 2. Describe in detail protein synthesis and the various post-translational modifications of proteins. II. Write notes on :

(10X6=60)

1. Describe the various stages of cell cycle with a note on its regulation. 2. Define mutation. Classify & describe the various types with examples. 3. Describe the types of DNA damage & repair mechanism. 4. Describe the mechanism of action of Type I hormones. 5. What are the various diagnostic & prognostic tests to be performed in a diabetic patient. 6. How is calcium homeostasis maintained in our body? 7. Describe in detail the structure , types and functions of Immunoglobulins. 8. CSF analysis and its application. 9. Estimation of Gamma Glutamyl Transferase(GGT). 10. Prenatal diagnosis of inborn errors of metabolism. *******

Dr. MGR Medical University M.Sc Biochemistry Molecular Biology.pdf

Page 1 of 3. [LA 0412] Sub. Code: 1203. M.Sc BIOCHEMISTRY DEGREE EXAMINATION. Candidates admitted from 2008-2009 batch. PAPER III – MOLECULAR BIOLOGY, CLINICAL BIOCHEMISTRY,. FUNCTION TESTS, ENDOCRINILOGY, IMMUNOLOGY, RECENT. ADVANCES IN BIOCHEMISTRY. Q.P. Code : 281203.

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