Microscopic Workshop for Label Free Live Cell Imaging of Single-Cell Dynamics in 3D? Look Inside Life using Nanolive!

+ WHEN? • Introductory Lecture: 10.00 a.m. - Wednesday 14th March 2018 • HANDS ON session: 13th to 20th March 2018 (on site registration) WHERE? BIOCEV, Imaging Methods Core Facility (IMCF) Address: Průmyslová 595, Vestec • Lecture - room U1.037 • HANDS ON session - room L1.008 • Coffee break after lecture NANOLIVE TECHNOLOGY • three-dimensional high spatial-temporal resolution imaging • (x, y:200nm; z:400nm; t:1.7sec) of transparent unlabeled specimens • labeling not required – post processing digital staining for 3D reconstruction • incubated controlled environment for long term time-lapse experiments possible using on stage incubator (available on workshop) • 3 Fluorescence channels to combine tomographic data with fluorescence markers (available on workshop) • excitation channels: DAPI (Semrock 392/23 BrightLine HC) / FITC (Semrock 474/27 BrightLine HC) / TRITC (Semrock 554/23 BrightLine HC) or Cy5 (Semrock 635/18 BrightLine HC)

» 3D Cell Explorer-fluo: A revolutionary Holo-tomographic Microscope to look instantly inside living cells in 3D without staining!«

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SAMPLE PREPARATION MANUAL: • cells grown on FluoroDish™ (3.5 mm) or fixed on glass coverslips • use a liquid mounting medium (e.g. PBS, culture medium etc.) - no dry samples • tissue sections (5-10 microns), remove the OCT14 th and WHEN or paraffin before mounting the slide

Holographic Free Microsc WHERE

PROGRAMME

CELLIM L

Luca Cla Michael

Wednesday February 14 13:00–14:00 Registration

14:00–15:00 Lecture - Principl 15:00–15:15 Coffee break

15:15–17:15 HANDS ON sessio

Thursday February 15 th

09:00–11:00 HANDS ON sessi 11:00–11:15 Coffee break

For more information write to Michaela Pluskalová, HPST, s.r.o. e-mail: [email protected]

11:15 –13:15 HANDS ON sessi

Capacity is limited. If you want to please contact petra.machackov