Product Data Sheet Recombinant Mouse IGF-I Catalog Number: 200-05

Product Description_____________________________________________________________________ Alternate Names: Somatamedin C, mechano growth factor, IGF-IA Accession Number: Q8CAR0 Description: Insulin-like growth factor I (IGF-I) is a growth factor that is produced by the liver. IGF-1 production is stimulated by growth hormone (GH). IGF-I binds the insulin-like growth factor 1 receptor (IGF1R) and the insulin receptor to stimulate systemic body growth. IGF-I is one of the most potent activators of the AKT signaling pathway, which stimulates cell proliferation and inhibits programmed cell death. Source: Genetically modified E.coli. Predicted MW: Monomer, 7.7 kDa (70 aa) AA Sequence: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*__________________________________________________________________ *Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements. Specification Method of Determination Acceptance Criteria Reducing and Non-Reducing SDS PAGE ≥95% Purity Kinetic LAL ≤1EUs/µg Endotoxin FDC-P1 cell proliferation ≤20 ng/mL; ≥ 5.0 x 10^4 units/mg Biological Activity

This product has also been tested with the following assay(s): MCF‑7 cell proliferation (typical ED50 is < 1 ng/mL)

Preparation and Storage_________________________________________________________________ Country of Origin: USA Shipping: Room temperature Storage Prior to Reconstitution: -20ºC Product Reconstitution: Sterile water at 0.1 mg/mL Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws. Expiration Date:  12 months from date of receipt when stored at -20 to -80 ºC as supplied.  1 month when stored at 4 ºC after reconstituting as directed.  3 months when stored at -20 to -80 ºC after reconstituting as directed.

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!