IV B.Tech I Semester Examinations,MAY 2011 METABOLIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. What are recalcitrant xenobiotic compounds? Explain general features of biodegradation of xenobiotic compounds. [8+8] [16]
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2. What is metabolism? How anabolism and catabolism integrated.
3. Explain various strategies to identify rate limiting step in a pathway.
[16]
4. What is metabolic pathway modeling? Explain software tools used for metabolic pathway modeling. [16]
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5. What are mutants? Explain methods for selective isolation of improved strains. [16] 6. Explain the metabolic pathway manipulations to improve the production of tryptophan. [16]
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7. What is genetic design? Explain how nature has produced a vast diversity of polyketides. [16] 8. Explain biodegradation of BTX mixtures? Explain role of metabolic mixtures.[16]
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Code No: 07A72312
R07
Set No. 4
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IV B.Tech I Semester Examinations,MAY 2011 METABOLIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Explain the metabolic regulation by regulation of enzyme concentration.
[16]
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2. What do you understand by feedback regulation? Explain this with special reference to amino acid biosynthetic pathways. [16] 3. Write a detailed note on: (a) Fed-batch Fermentation
[8+8]
or
(b) Continuous fermentation.
4. Briefly explain different types of pathway manipulations to improve fermentation. [16] [16]
6. Write about various producers of secondary metabolites.
[16]
7. How bioinformatics fortified metabolic engineering?
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5. Explain various applications of metabolic engineering in pharmaceuticals.
8. Briefly explain two fundamentally different ways in which a cell might control the rate of an enzyme reaction. [16]
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Code No: 07A72312
R07
Set No. 1
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IV B.Tech I Semester Examinations,MAY 2011 METABOLIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Briefly out line the procedure for selection of microorganism producing a compound. [16]
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2. How can metabolic pathways genetically controlled explain with any two examples? [16] 3. Explain in detail different strategies that can be adopted for maximizing the yield of secondary metabolite. [16]
5. Write short notes on:
or
4. Role of bioinformatics in metabolic pathway modeling.
[16]
(a) Origin of capacity to degrade xenobiotics by microorganisms
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(b) Use of mixed microbial populations.
[8+8]
6. Explain metabolic flux analysis of citric acid fermentation of Candida lipolytica proposed by Aiba and Matsuoka. [16] 7. Write short notes on:
(a) aerobic biodegradation of pollutants
(b) anarobic biodegradation of pollutants.
[16]
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8. Explain the metabolic pathway manipulations to improve the production of 1, 3 propanediol. [16]
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Code No: 07A72312
R07
Set No. 3
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IV B.Tech I Semester Examinations,MAY 2011 METABOLIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Explain the amino acid synthesis pathways regulation at whole cell level.
[16]
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2. Role of metabolic engineering in lactose and whey utilization in dairy industry.[16] 3. What are the ideal characteristics of strains? Write about different approaches to improve the microbial strain. [16] 4. Write short notes on the following:
or
(a) How specific rates and yields are related?
(b) Explain the calculation of yields and specific rates.
[8+8]
5. How the performance of the cell is achieved? Explain the methodology behind it. [16]
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6. Briefly explain various methodologies and their applications in metabolic engineering. [16] 7. What are precursor effects? Briefly explain the regulation of secondary metabolic pathways. [16]
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8. Explain briefly how radiolabel materials are utilized in experimental determination of metabolic flux. [16]
Briefly explain two fundamentally different ways in which a cell might control the ... Explain metabolic flux analysis of citric acid fermentation of Candida lipolytica.
(a) What is Radix-64 format? Explain how both PGP and S/MIME perform the · Radix-64 conversion is performed. (b) Describe the five principal services that ...
Farm in Maharashtra, supplied with water from a dug well pumping for 4 h a day, are given below. Determine the required discharge. [16]. 7. AjntuWorld.in ...
... carry equal marks · ***** · 1. List eight major functions of an exhaust nozzle. .... plane. Show the internal layout for swallowed air to reach the engine. Explain.
Enumerate difference between growth and non-growth associated product kinetics. [16] · 7. Write a brief note on biosensors, and describe how they are used for ...
(b) A turbine is to operate under a head of 30 m at 300 r.p.m. The discharge is ... 2. (b) An inward flow reaction turbine has an external diameter of 1 m and its.
(a) Discuss the response of high pass RC circuit for square wave input. (b) A 10Hz square wave is fed to an amplifier. Calculate and sketch the output wave ...
[16] · 8. With a neat sketch discuss the working procedure of neutron radiography, and also · give applications, advantages and limitations of this method. [16] · *****. 1 ... 5. (a) Give the limitations of x-ray diffraction method and ultrasonic met
tetrachloride during indirect chlorinate of TiO2 at 1020 0C · [16] · TiO2(g) + 2Cl2(g) = TiCl4(g) + O2. 8. (a) How do the interstitial elements affect mechanical properties of commercially · pure titanium? (b) Why is 0.2 % Pd added to titanium? What
register machine.(Hint: Use FNS). [8+8] · 5. (a) Explain the strassen's matrix multiplication · (b) Derive the time complexity for binary search. [8+8] · 6. (a) Find an ...
Variance · (b) The mean and variance of a binomial distribution are 2 and 8 / 5. Find ... marks were normally distributed with mean 70 and standard deviation 15.
Computer Science And Engineering · Time: 3 hours · Max Marks: 80 · Answer any FIVE Questions · All Questions carry equal marks · ***** · 1. (a) Distinguish between a packet-filtering router and a stateful inspection firewall? (b) âOne way to secure
1. With the help of a neat diagram indicating the various geometric elements of a · traffic rotary ... sight distance. What is the need to have SSD and factors.
2. Explain the alpha, delta, theta and beta waves emerging from the brain. [16] · 3. (a) Explain in detail on the isolation amplifiers used in medical field and its need ... Discuss the generation and propagation of action potential in a cell. [16] ·
(a) Steam pressure in a reactor heating coil. (b) Flow rate of reactants into a polymerization tank. (c) Flow of effluent from a waste water treatment holding tank into a river. (d) Flow of cooling water to a distillation condenser. [16] · 6. (a) Def
(a) State Newton's law of viscosity and give any four examples of its applications. (b) An oil of viscosity 8 poise is used for lubrication between a shaft and sleeve.
(a) Off - peak load is zero ... load current and p.f 0.1. capacitors are supplied to make the line power factor ... (b) The annual peak load of substation is 3500kW.
Set No. 2. (a) Construct a systematic (15,2) code using the generator polynomial .... taken. The bit rate is 'rb' and the channel noise has a PSD Gn(f) given by.
Discuss the principle of measuring the hardness of Heat Affected Zone using Jominey · hardenability test? .... (b) What is post weld heat treatment? Describe the ...
(a) State Darcy's law. Discuss the assumptions and limitations. .... 1. (a) State Stoke's law and explain its limitations as applicable to the sedimentation · analysis.
[4+4+4+4] · 4. Add a new method in the base class of Shapes.java that prints a message, but don't .... (d) Dynamic binding and message passing. [4+4+4+4].
Discuss clustering and solubility of liquid mixture. [16] · 3. Define: ... no adsorption at the pore wall and for the case with monolayer adsorption at the · pore wall.
