DOC # CS-100344 REV 0

Product Insert CADMIUM SULFATE FLUORIDE ACRIFLAVINE TELLURITE AGAR (CFAT) Products: AS-6424

Cadmium Sulfate Fluoride Acriflavine Trypticase Agar (CFAT)

4 plates / pkg

Intended Use CFAT has been formulated for the enhanced isolation of Actinomyces naeslundii and Actinomyces viscosus from mixed culture.

Summary CFAT is made of Tryptic Soy Broth and dextrose which form the nutritive base of this medium. This medium is supplemented with cadmium sulfate, sodium fluoride and neutral acriflavine to enhance the isolation of A. naeslundii and A. viscosus. Colonies of Actinomyces spp. are cream to slightly greenish in color, entire, convex or raised and opaque on this medium. This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Formula (g/L) Casein Soy Broth Dextrose Agar Cadmium Sulfate Sodium Fluoride Neutral Acriflavine Basic Fuchsin Sheep Blood Potassium Tellurite DI Water

30.0 g 5.0 g 15.0 g 0.013 g 0.08 g 0.0012 g 0.00025 g 50.0 mL 1.0 mL 1.0 L

Final pH 7.0 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

Precautions For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic techniques should be observed when using this product. This product is for use only by properly-trained and qualified personnel. Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life Storage: Upon receipt, store at room temperature (13°C - 27°C) in original container until use. Avoid overheating or freezing. Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container. Shelf Life: 90 days from date of manufacture.

Procedure Specimen Collection: Specimens for anaerobic culture should be protected from oxygen during collection, transportation and processing. Consult appropriate references for detailed instructions concerning collection and transportation of anaerobes. Methods for Use: CFAT agar should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material. Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37oC for 2-7 days. Additional periods of incubation may be necessary to recover some anaerobes. Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

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Anaerobe Systems 15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031 http://www.anaerobesystems.com

DOC # CS-100344 REV 0

Materials Required But Not Provided Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations CFAT agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present. Consult reference materials for additional information.

Quality Control This medium should support good growth of some Actinomyces spp. found in clinical infections. The following organisms are routinely used for quality control testing at Anaerobe Systems. Organism Tested Fusobacterium nucleatum Actinomyces viscosus Bacteroides fragilis Enterococcus faecalis

ATCC # 25586 43146 25285 29212

Results No Growth Growth Growth Growth

Time (Hours) 48 48 48

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user. If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours. If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition. Organism A. viscosus F. nucleatum B. fragilis

Expected Growth 48 hrs No Growth 48 hrs

Physical Appearance: CFAT should appear opaque maroon red in color.

References 1.

Dowell, V. R., Jr. and T. M. Hawkins. 1974. Laboratory Methods in Anaerobic Bacteriology. CDC Laboratory Manual. USDHEW C. D. C. Atlanta, GA 30333.

2.

Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R. 1992. Principles of Practices of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA 94002.

3.

Holdeman, L. V., F. P. Cato and W. E. C. Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute and State University. Blacksburg, VA 24061.

4.

Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold. 2002. Wadsworth – KTL Anaerobic Bacteriology Manual. Star Publishing Co. Belmont, CA 94002.

5.

NCCLS. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition. (2004). NCCLS document M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

Revision Date: 8/28/15

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Anaerobe Systems 15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031 http://www.anaerobesystems.com