DOC # CS-100356 REV 0

Product Insert TRYPTIC SOY SERUM BACITRACIN VANCOMYCIN AGAR (TSBV) Products: AS-648

Tryptic Soy Serum Bacitracin Vancomycin Agar (TSBV)

4 plates / pkg

Intended Use TSBV is an enriched selective medium for the isolation and presumptive identification of Aggregatibacter actinomycetemcomitans, (formerly known as Actinobacillus actinomycetemcomitans).

Summary TSBV medium contains bacitracin and vancomycin at a concentration that inhibits most gram-positive and most gram-negative anaerobes, except for Aggregatibacter actinomycetemcomitans among others. This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Formula (g/L) Pancreatic Digest of Casein Soy Peptone Sodium Chloride Agar Yeast Extract Bacitracin Vancomycin Horse Serum DI Water

15.0 g 5.0 g 5.0 g 15.0 g 1.0 g 75.0 mg 5.0 mg 100.0 mL 1.0 L

Final pH 7.1 +/- 0.2 at 25 degrees C. Final weight 16.0 g +/- 1.6.

Precautions For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic techniques should be observed when using this product. This product is for use only by properly-trained and qualified personnel. Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life Storage: Upon receipt, store at room temperature (13°C - 27°C) in original container until use. Avoid overheating or freezing. Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container. Shelf Life: 90 days from date of manufacture.

Procedure Specimen Collection: Specimens Specimens for anaerobic culture should be protected from oxygen during collection, transportation and processing. Consult appropriate references for detailed instructions concerning collection and transportation of anaerobes. Methods for Use: TSBV should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material. Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37oC for 18 – 48 hours. Additional periods of incubation may be necessary to recover some anaerobes. Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

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Anaerobe Systems 15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031 http://www.anaerobesystems.com

DOC # CS-100356 REV 0

Materials Required But Not Provided Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations TSBV will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from clinical specimens to assure growth of all species present. Consult reference materials for additional information.

Quality Control This medium should support good growth of A. actinomycetemcomitans found in clinical infections. The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. Organism Tested Aggregatibacter actinomycetemcomitans Actinomyces viscosus Fusobacterium nucleatum Propionibacterum acnes Clostridium difficile Enterococcus faecalis

ATCC #

Results

29522 43146 25586 6919 9689 29212

Growth No Growth Growth No Growth No Growth No Growth

Time (Hours) 24 – 48 24 – 48

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user. If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours. If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition. Organism A. actinomycetemcomitans A. viscosus F. nucleatum E. faecalis

Expected Growth 24-48 hrs Inhibited 24-48 hrs Inhibited

Physical Appearance: TSBV should appear translucent yellow in color.

References 1.

Dowell, V. R., Jr. and T. M. Hawkins. 1974. Laboratory Methods in Anaerobic Bacteriology. CDC Laboratory Manual. USDHEW C. D. C. Atlanta, GA 30333.

2.

Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R. 1992. Principles of Practices of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA 94002.

3.

Holdeman, L. V., F. P. Cato and W. E. C. Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute and State University. Blacksburg, VA 24061.

4.

Slots, J. 1982. Selective Medium for Isolation of Actinobacillus actinomycetemcomitans. Journal of Clinical Microbiology. April 1982, p. 606 – 609.

5.

Somer-Jousimies, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold. 2002. Wadsworth – KTL Anaerobic Bacteriology Manual. Star Publishing Co., Belmont, CA 94002

6.

NCCLS. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition. (2004). NCCLS document M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

Revision Date: 9/1/15 Page 2 of 2

Anaerobe Systems 15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031 http://www.anaerobesystems.com