Dr. Chithra Manisseri Assistant professor Dept. of Plant Science Central University of Kerala, Riverside Transit Campus, Padannakkad, Nileshwar, 671314, Kasaragod, Kerala, INDIA

E-mail:[email protected] [email protected] Phone: 8129733003

Residence Address: Quarters No: 5, Pathayappura, Kovvalpally, Kanhangad South (PO) Kasaragod 671315

RESEARCH INTERESTS: Cell wall polymers. Hemicellulases. Bioenergy. Bioactive components and nutrition RESEARCH FOCUS: My area of research is focused on plant cell wall polymers and their importance in bioenergy and as bioactive components. Either to modify or deconstruct the cell wall, a detailed knowledge on the structure and composition of the wall components is needed. The recalcitrance of the lignocellulose plays a vital role in determining the biofuel yield. Plant cell wall composition is species, tissue/cell specific and depends on environmental conditions, all having impacts on biofuel production. A detailed information will be obtained by screening some of the potential feedstocks in India which will be useful for the enhanced biofuel production. Special emphasis will be given for deacetylation and deferuloylation and optimizing the pretreatment processes. I am also interested to look at the key roles of the cell wall polymers in human nutrition EDUCATION 2003-2008

Ph.D. Biotechnology Central Food Technological Research Institute, (CFTRI)/University of Mysore, India

2000-2002

MSc Plant Science University of Calicut, Kerala, India

PhD thesis: ‘Xylanase from ragi malt and it’s use in the preparation of xylooligosaccharides from cereal brans’ MSc thesis: In vitro morphogenic studies on medicinally important Rotula aquatica Lour. and Kaempferia galanga L

Positions and Research Experiences: th

4 Oct.2016- Present

Assistant professor, Dept. of Plant Science, Central University of Kerala

June 2016 -Oct.2016

UGC-Assistant Professor (UGC-FRP)- Dept. of Microbial Biotechnology, Bharathiar University, Coimbatore

2015 April-2015 Dec

Postdoctorate fellow- Institute of Plant and Microbial Biology, Academia Sinica, Taiwan R.O.C.

2014 August- 2015 March

Guest researcher- Institute of Plant and Microbial Biology, Academia Sinica, Taiwan R.O.C.

2010 Feb- 2012 Dec

Postdoctorate fellow- University of Hamburg, Germany

2008 April- 2009 Dec

Postdoctorate fellow- Joint BioEnergy Institute (JBEI)/ Lawrence Berkeley National Laboratory (LBNL) California, USA.

PUBLICATIONS

1. Lin F, Manisseri C, Fagerström A, Peck ML, Vega-Sánchez ME, Williams B, Chiniquy DM, Saha P, Pattathil S, Conlin B, Zhu L, Hahn MG, Willats WGT, Scheller HV, Ronald PC, Bartley LE (2016). Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development. Plant and Cell Physiology, pcw125. doi: 10.1093/pcp/pcw125 . 2. Meineke T, Manisseri C, Voigt CA (2014). "Phylogeny in defining model plants for lignocellulosic ethanol production: a comparative study of Brachypodium distachyon, wheat, maize, and Miscanthus giganteus leaf and stem biomass". PLoS ONE 9(8): e103580. doi:10.1371/journal.pone.0103580 3. Ellinger D, Glöckner A, Koch J, Naumann M, Stürtz V, Schütt K, Manisseri C, Somerville SC, Voigt CA (2014). Interaction of the Arabidopsis GTPase RabA4c with its effector PMR4 results in complete penetration resistance to powdery mildew. Plant Cell, Published online doi: http://dx.doi.org/10.1105/tpc.114.127779 4. Bartley LE, Peck ML, Kim SR, Ebert B, Manisseri C, Chiniquy DM, Sykes R, Gao L, Rautengarten C, Vega-Sánchez ME, Benke PI, Canlas PE, Cao P, Brewer S, Lin F, Smith WL, Zhang X, Jentoff RE, Foster SB, Zhou J, Ziebell A, An G, Scheller HV, Ronald PC (2013). Overexpression of a BAHD Acyltransferase, OsAt10, alters rice cell wall hydroxycinnamic acid content and saccharification. Plant Physiology 161: 1615–1633. 5. Ellinger D, Naumann M, Falter C, Zwikowics C, Jamrow T, Manisseri C, Somerville SC, Voigt CA (2013). Elevated early callose deposition results in

complete penetration resistance to powdery mildew in Arabidopsis. Plant Physiology 161: 1433–1444

6. Manisseri C, Gudipati M (2011). Prebiotic activity of purified xylobiose obtained from ragi (Eleusine coracana, Indaf-15) bran. Indian Journal of Microbiology, 52: 251-257. 7. Yin L, Verhertbruggen Y, Oikawa A, Manisseri C, Knierim B, Prak L, Jensen JK, Knox JP, Auer M, Willats WGT, Scheller HV (2011). The cooperative activities of CSLD2, CSLD3, and CSLD5 are required for normal Arabidopsis development. Molecular Plant, 4: 1-14. 8. Arora R, Manisseri C, Li C, Ong M, Scheller HV, Vogel K, Simmons BA, Singh S (2010). Monitoring and analyzing process streams towards understanding ionic liquid pretreatment of switchgrass (Panicum virgatum L.)" .Bioenergy Research, 3:134-145. 9. Manisseri C, Gudipati M (2010). Bioactive xylo-oligosaccharides from wheat bran soluble polysaccharides’- LWT- Food Science and Technology, 43: 421-430. 10. Oikawa A, Joshi HJ, Rennie EA, Ebert B, Manisseri C, Heazlewood JL, Scheller HV (2010). An integrative approach to the identification of arabidopsis and rice genes involved in xylan and secondary wall development. PLoS ONE, 5: e15481. 11. Li C, Knierim B, Manisseri C, Arora R, Scheller HV, Auer M, Vogel KP, Simmons BA, Singh S (2009). Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification. Bioresource Technology, 101: 4900-4906. 12. Manisseri C, Gudipati M (2008). ‘Characterization of purified xylanase from finger millet (Eleusine coracana-Indaf 15) malt’ – European Food Research and Technology, 227: 587-597. 13. Manisseri C, Gudipati M (2008). An improved method for obtaining xylanase from Finger millet (Eleusine coracana, Var.‘Indaf-15’) malt’. Journal of Food Science and Technology, 45: 166-169. 14. Manisseri C, Martin KP, Sunandakumari C, Madhusoodanan PV (2005). Protocol for rapid propagation and to overcome the delay of rhizome formation in field established in vitro derived plantlets of Kaempferia galanga L., Scientia horticulturae, 104: 113-120. 15. Manisseri C, Martin KP, Sunandakumari C, Madhusoodanan PV (2005). Somatic embryogenesis, encapsulation and plant regeneration on Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. In Vitro cellular and Developmental Biology Plant, 41: 28-31.

16. Manisseri C, Martin KP, Sunandakumari C, Madhusoodanan PV (2005). Silver nitrate induced rooting and flowering in vitro on rare rhoeophytic woody medicinal plant Rotula aquatica Lour, Indian Journal of Biotechnology, 3: 418-421. 17. Martin KP, Sunandakumari C, Manisserri C, Madhusoodanan PV (2005). Influence of auxins in direct in vitro morphogenesis of Euphorbia nivulia, a lectinaceous medicinal plant. In Vitro Cellular and Developmental biology Plant, 41: 314-319. 18. Sunandakumari C, Martin K.P, Manisseri C, Madhusoodanan PV (2004). Rapid axillary bud proliferation and ex vitro rooting of herbal spice, Mentha piperita L. Indian Journal of Biotechnology, 3:108-112. PATENT- 1

1. Manisseri C, Gudipati M (2006). A patent entitled ‘A process for the preparation of xylanase from ragi malt’.DEL No: 326/DEL/2006 . PRESENTATIONS IN NATIONAL/ INTERNATIONAL SYMPOSIA - 13

1 Manisseri C, Arora R, Benke P, Li C, Smith M, Sun L, Ong M, Scheller HV, Vogel K, Simmons BA, Singh S. Understanding deferuloylation and delignification in bioenergy crops during ionic liquid pretreatment. Presented in Cell wall meeting held at Porto, Portugal between 25-30 July 2010. 2 Meineke T, Manisseri C, Voigt CA. Comparative studies of biomass fermentation of energy crops and its model plant Brachypodium distachyon. Presented in Cell wall meeting held at Porto, Portugal between 25-30 July 2010. 3 Manisseri C, Arora R, Li C, Smith M, Sun L, Ong M, Benke P, Schiller HV, Vogel K, Simmons BA, Singh S. Compositional analysis of lignin in bioenergy crops and depolymerization via ionic liquid pretreatmet. Presented for SIM meeting held at San Francisco during 3-6 th May 2009. 4 Yin L, Manisseri C, Jensen JK, Willats W, Scheller HV. CslD5 is β-mannan synthase involved in vascular development. Presented in Plant Cell Wall Gordon Conference, Bryant University, Smithfield, RI, USA, August 2-7, 2009. 5 Manabe Y, Manisseri C, Orfila C, Smith M, Çetinkol OP, Holmes B, Heazlewood JL, Scheller HV. Identification of genes involved in acetylation of cell wall polysaccharides in Arabidopsis thaliana. Presented in JGI meeting, California, March 25, 2009.

6 Knoch E, Manisseri C, Verhertbruggen Y, Geshi N, Scheller HV. Identification and characterization of three putative glycosyltransferases involved in the biosynthesis of arabinogalactan proteins. Presented in Synthetic biology workshop at Berkeley, California, 23-25 July 2009 7 Bharadwaj R, Manisseri C, Scheller HV, Singh S, Singh AK. Microfluidic approaches to glycan analysis. Presented at Annual retreat at JBEI, California Feb 811, 2009 8 Manisseri C, Arora R, Jorgens D, Auer M, Scheller HV, Simmons BA, Singh S. Understanding ionic liquid pretreatment of lignocellulosic biomasses. Presented during Annual retreat at JBEI, California, Feb 8-11, 2009. 9 Bartley L, Manisseri C, Conlin B, Chiniquy D, Harholt J, Scheller HV, Ronald PC. Analysis of putative feruloyl transferase transcript levels and cell wall composition during rice development. Presented for GTL meeting at Maryland, Feb 8-11, 2009. 10 Li C, Arora R, Manisseri C, Vogel K, Scheller HV, Simmons BA, Singh S. A comparative study of dilute acid and ionic liquid pretreatment of biomass and model lignocellulosics. Presented for SIM meeting held at San Francisco during 3-6 th May 2009. 11 Manisseri C, Gudipati M. Purification and structural characterization of bioactive xylo-oligosaccharides from cereal brans’- Presented in CARBO-XXI, held at Delhi University, New Delhi, between 26- 29 November, 2006. 12 Manisseri C, Gudipati M. Kinetic studies of a purified xylanase from ragi maltPresented in the 74th Annual Meeting, Society of Biological Chemists (India) held at Central Drug Research Institute, Lucknow, India between 7- 10 November 2005. 13 Manisseri C, Gudipati M. Purification and partial characterization of xylanase from finger millet malt’. Presented in colloquium on novel proteins in nutrition and health organized by the Solae Company and Central Food Technological Research Institute, Mysore, 22nd March 2005. INVITED TALK Invited to give a talk in Biozentrum Klein flottbek, University of Hamburg in 2009 September on "Understanding plant cell walls: A potential key to biofuel production from biomass".

REVIEWER Reviewer for ‘Rice”-Springer open journal.

AWARDS/RECOGNITIONS CSIR-JRF-NET Nationally selected for UGC-Assistant professor position in Biological Sciences under the Faculty Recharge Programme (UGC-FRP), 3 rd cycle.