*PG834*

PG – 834

II Semester M.Sc. Degree Examination, July/August 2013 (Semester Scheme) (NS) BIOCHEMISTRY BC-202 : Biochemical Techniques Time : 3 Hours

Max. Marks : 80

Instruction : Answer question no.1 and any five of the remaining. 1. Answer any ten of the following :

(10×2=20)

a) Give the principle of paper chromatography. b) How is matrix for PAGE generated from monomers ? c) Give the principle of organ perfusion studies. d) Mention the types of ion-exchangers used in biochemical studies. Give one example for each. e) Give the principle of thermal conductivity detectors used in GLC. f) A solution of p-nitrophenol showed an absorbance of 2.4 using 1.0 cm cuvett at 410 nm.Calculate the amount of p-nitrophenol present in 6.0 ml of this solution. (Molar extinction coefficient of riboflavin at 410 nm is 1.2 ×105 M–1cm–1) g) Why are spacers used in affinity chromatography ? h) Distinguish between stable isotopes and radioisotopes. Give one example for each. i) Give the applications of IR spectroscopy. j) What is Svedberg constant ? k) What is chromatofocussing ? l) What is dialysis ? P.T.O.

*PG834*

PG – 834

2. a) Give the principle of gel permeation chromatography. Explain how this technique is used to establish the oligomeric nature of proteins. b) Describe the components of HPLC and give the applications of this technique. (7+5=12) 3. a) Describe the experimental set up to determine the molecular weight of proteins using sedimentation velocity method. b) Explain the strategies employed in whole animal studies.

(7+5=12)

4. a) Give the construction and applications of UV-Vis spectrophotometer. b) Explain the principle, procedure and applications of Isoelectrophoresis. (6+6=12) 5. a) Explain the principle and limitations of the use of liquid scintillation counter for the measurement of radioactivity. b) Explain the procedure used in developing affinity matrices.

(6+6=12)

6. a) Explain the principle and procedure and application of PAGE. b) Explain the procedure used in preparation of TLC plates. Explain how resolved substances are detected in this technique. (6+6=12) 7. Write notes on any three of the following : a) Cell culture technique b) Counter Current Distribution technique c) Ultra filtration technique d) Warburg respirometer.

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(3×4=12)