How Can DNA Replication Be Modeled? Objectives: Build a model of DNA using colored paper clips and use the model to illustrate how semiconservative replication takes place. Compare the actions of DNA polymerase to DNA helicase. Summarize how semiconservative replication reduces errors in copies Procedure: You will construct a doublestranded Human Growth Hormone (hGH) gene using colored paperclips. Adenine (A) = black Thymine (T) = white Cytosine (C) = red Guanine (G) = geen Step 1: Build Human hGH DNA Link the appropriate clips togather to create 2 stands of hGH according to the following modified sequence. The actual gene consists of 573 bases. Remember, to follow the basepair rule.
A‐A‐G‐C‐T‐T‐A‐T‐G‐G‐T‐C‐C‐C‐G‐G‐A‐C‐G‐A‐A‐G‐C‐T Step 2: Unzip DNA Replication of DNA is called semiconservative because the process saves, or conserves, half of the original strand. Helicase enzymes in the cell first UNZIP the DNA molecule. Separate the two sides of the DNA molecule you built to model this process. Step 3: Build New Strands DNA polymerase builds a new half on each side of the unzipped strand, following the base pair rule. Add new paperclips of the appropriate color to each side of the unzipped molecule. When finished, you should have two complete doublestranded DNA molecules now.
Discussion: 1. How would this method or replication reduce errors (mutations) in newly constructed DNA? 2. Compare DNA helicase to DNA polymerase. What are their roles in replication? 3. What is the basepair rule? How does it come into play during replication?
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2.7 DNA replication, transcription and translation. Essential Idea: Genetic information in DNA can be accurately copied and can be translated to make the proteins needed by the cell. The image shows an electron micrograph of a Polysome,. i.e. multipl
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these words (semiâhalf; conserveâto keep), explain why DNA replication is called. semi-conservative. Page 3 of 4. 18 DNA Structure and Replication-S.pdf.
The five diagrams in the table below illustrate the main parts of the process of DNA replication which you need to understand for SL. Look at each diagram and explain how each image is important in the process. Image. Important in DNA replication bec
Page 1 of 5. DNA Structure and Replication 1. DNA Structure and Replication. How is genetic information stored and copied? Why? Deoxyribonucleic acid or ...
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They established the structure as a double helix. The sugar. and phosphates make up the "backbone" of the DNA. molecule. DNA Structure: DNA is composed of monomers called nucleotides. Each. nucleotide consists of: 1. a phosphate. 2. a sugar (deoxyrib
new strands. 1. DNA Helicase unwinds and. unzips the DNA strands at the. replication fork. 2. DNA Polymerase adds the. complementary nucleotides to the.