St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001 PMB1015 - GENETIC ENGINEERING Time : 3 hrs Max Marks :75 SECTION – A (5X5=25) Answer any FIVE Questions 1. Briefly explain about Sanger’s method of DNA sequencing. 2. Multiplex PCR assay – give a brief note. 3. Explain about oligonucleotide directed mutagenesis. 4. Signify the Microprojectiles for transfections into living cells. 5. Give detailed information on: (i) Binary vectors and (ii) Shuttle vectors with diagrams. 6. Briefly explain about the problems encountered in cloning of eukaryotic genes in to prokaryotes. 7. Illustrate – Superbug. 8. Briefly explain DNA chips. SECTION – B (5X10=50) Answer any FIVE Questions 9. What is gene sequencing? Explain about Maxam-Gilbert method of gene sequencing. 10. Elucidate the method-Shot-gun sequencing approach.
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11. How will you clone the PCR products and interpretate in detail? 12. What is the methodology for the construction of chimaeric proteins? Explain. 13. Agrobacterium in genetic engineering - Justify. 14. ‘Gene transfer’ – explain. Discuss about various types of gene transfer technologies. 15. How far the genetic engineering play vital role in recombinant insulin production? 16. Describe the various applications of recombinant technology
9. What is gene sequencing? Explain about Maxam-Gilbert method of. gene sequencing. 10. Elucidate the method-Shot-gun sequencing approach. Page 1 of 2 ...
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