Albanian j. agric. sci. 2017; (Special edition)

Agricultural University of Tirana

(Open Access)

RESEARCH ARTICLE

IBA for rooting influence of some varieties of pomegranate (Punica granatum L). TATJANA KOKAJ*, ADRIATIK ÇAKALLI, HAIR ISMAILI Agriculture University of Tirana/ Institute of Plant Genetic Resource Corresponding author e-mail: [email protected]

Abstract Pomegranate (Punica granatum L ) belongs to the family Punicaceae and is widespread in Albania. This research was intended, to discover the effect of AIB (indole acid buturik), for development of meristems, rooting of three main forms pomegranate, Shg: cv 11/1; 13/1 Shg, Shg 14/1. The method was application with plant material obtained from the wood scion bare pieces, in situ, in Lezha, Shkodra and Tepelene in late January - early February time. The pieces scions have been 10-12 cm dimensions. and eventually they settled in two variant: (i) no treatment control, (ii) 2 hours 1000ppm IBA to be treated about 1.5 cm at the base of the pieces. The pieces are left to dry by the alcohol vapors and then planted in warm bank with perlite substrate. The hygroscopic watering which realisated once time for every day with 1 minute. Results of pieces scions rooting after 90 days have been visible changes between forms and two variants (Cv = 21.3). In generally, three forms have been percent rotting about 90% versus 70 percent in terms control. The percentage of indigenization was in the varieties in correlation, r = 0.82 and r = IBA presence of 0.89. Keywords: screenhouse, Punica granatum, sample, plot, perlit

was increase cultivation interes for fresh fruit from farmer and market request.

Indroduction Pomegranate (Punica granatum) is older tree, has been cultivated since antiquity, has a older history, founded in report In literature, art, culture, religion, etc. Is tree

Materials and methods

tropical and subtropical region. Pomegranate tree was

The aim. of this experiment was rooting of

widelyspread in Mediteranean, like Mediteranean

cutts pomegranate on three different variants from

climatic condition.Area with relative humidity or rain

different region condition.

are not suitable for its cultivation, as fruits produced under such conditions tendend to taste less sweet.

Objective 1: The

rooting on screnhouse

condition in three way and three variants with five

Pomegranate (Punica granatum L) belongs to the Punicaceae, family, and originates in Central Asia. Poliphenol are rich in the pomegranate, which mainly

repeat. Objective 2: This genetic material must planting on germplasm field.

include ellagitamin, flavonoid and phenolic acid.

The study was conducted in the period 2013 -

There are numerous human health benefits of

2016. The cutts were collected in the country of

consuming



origin, in three countries by different characteristic

inflammatory. In our country founded in lowland sea

climatic condition, those Varieties has been study

and in some interior regions of the country.side.

before years ago, characterized for morphological

Pomegranate has becomes one of the most important

character. It used two ways rooting, with a nebulizim

fruits in the world and in our country, the last year

and with a black nylon bags. V-1 slips were cut 15-20

pomegranates,

such

as

anti

cm above the bud, they entered the hormonal IBA 133

Kokaj et al., 2017

solution was left to dry for a few minutes and then

V1 treated with IBA (Indole Acid Indobutirik) at

planted at the table and perlite. At the time of planting

concentrations 1000 ppm at the table of the perlit is a

greenhouse temperature was 18-19 C. When the

very good rooting, 95%, time rooting is the fast,

temperature began to rise above 20 C began to

staying more than 60 days has made kallusimi to be

activate slips, they began the first signs that

the best and to stay rooting natural stresses. And these

indigenization. When the outside temperature began

are independently saplings planted in bags for

to rise over 25 °, then began watering the plants

tranpiantim to permanent planting in the area of

through pulvorization, found that mediates ores. But

germplasm.

V 2 and V 3 were planted in bags filled with perlite,

As regards the second variant in nylon bags

and peat land. The dimensions of cuttings 20-25 cm. It

with soil, peat and little sand, they have rooted more

was placed in the bag. With increase the temperature

slowly, with 50 % rooting up to 45%, the time of the

out side screenhouse and inside screenhouse began

rooting of slower, with smaller dimensions the length

their irrigation. The data were processed statistically.

of the root and no of roots and dimensions of length

Results and disscusion

off shoot against the first variant. Likewise variant and V 3 with small dimensions to V1. From anylisis

According to the final results, the rooting of

statistic was resulted such is:

varieties in terms of the greenhouse, it is noted that Tabele 1: Analysis of Variance for the number of roots, the roots length and pomegranate of shoot length variants multiply in three variants Source Number of rotting Error C. Total Length rotting Error C. Total Length shoots Error C. Total

DF 2

Sum of Squares 1.9223333

Mean Square 0.961167

12 14 2 12 14 2 12 14

6.7398000 8.6621333 267.25552 4.50552 271.76104 392.83612 11.53232 404.36844

0.561650

F Ratio

Prob >

1.7113

0.2219

133.628 0.375

355.9041

<.0001*

196.418 0.961

204.3836

<.0001*

Analysis of the data proved the main changes

expressed the level of R with differences acceptable.

to rooting indexes pomegranate (Punica granatum).

Standard digression had small-scale variability within

Analysis of five repeat represent the number of

their repeat from average and was unnecessary to

variables to the roots, to root length (HR), the length

compare to the variation between them. In general

of the shoot (HL), have proven statistical homogeneity

hypothesis of the influence of variants of application

and their authenticity. Statistical analyzes have well

over index achieved is verification because in any

expressed the propagation of variable amplitudes and

case F ration> FTeorike.

Table 2: Analysis of Variance Means and and Std Deviations for roots number, roots length and shoots pomegranate Variant V1 V2 V3

N. Roots 1.51±0.44 a 2.04±0.66 a 2.38±1.02 a

Gj. Length shoots Roots 12.2±0.75 a 13.96±1.30 a 3.88±0.43 b 3.53±1.04 b 2.72±0.59 c 2.72±0.27 b

134

Lower 95% Prob > F 0.9549 <.0001* 1.2169 <.0001* 1.1103 <.0001*

IBA for rooting influence of some varieties of pomegranate (Punica granatum L).

Figure 1: Variance Analysis of the length of root in application for three varieties multiplication of pomegranate (Punica Granatum

Figure 2: Variance Analysis of the length roots by three options to apply for multiplication of pomegranate (Punica granatum

Figure 3. Variance Analysis of length shoots (cm) by three variants application for multiplication pomegranate (Punica granatum)

135

Kokaj et al., 2017

The average number of roots was 1.97, and

frequency Std.Dev 0.52 and 0.43 to 0.57, the length of

1:51 frequency to 2.3, showed no statistical difference

the root is visible changes between three variant tested

between the averages of variants (V1 ~ V2 ~ V3) by

for Prob> F <.0001 * because F/Factik is 355.9. First

Means test for all pairs comparisons using Tukey-

variant changing significantly between V2 and V3 and

Kramer. He confirmed the average 0.71 and frequency

level differences are within the limits of standard

Std.Dev 0:44 to 1:02, has resulted in the number of

deviation and have high reliability.

roots no apparent changes between three variants

The average length of the shoot (branch) was

tested for Prob> F <.0001 *. Level differences are

6.73 cm with a frequency between 2.7 to 13.9cm.

within the limits of standard deviation and high

Variants averages each changing between each other

reliability.

in order to visible. V1 has the greatest length of the

The average length of roots was 6.23 cm, and

branch in the order (V1> V2 ~ V3), V2 and V3 do not

frequency variations between 2.7 to 12.2cm. Data

have differences between them. Level differences are

between variants result in visible changes to statistical

within the limits of standard deviation and have high

variations between the averages (V1> V2> V3), for all

reliability and the value of each repetition are

pairs analyzed using Tukey-Kramer. The average

positioned within the contour of plot it credible.

Table: 3 Analysis of Variance Source Variant

DF

Sum of Squares

Mean Square

F

Prob > F

2

7843.3333

3921.67

37.5279

<.0001*

Error C. Total

12 14

1254.0000 9097.3333

104.50

Table: 4 Means and Std Deviations Level V1 V2 V3

Rooting % 87.0±4.47 a 39.0±3.31 b 38.0±16.8 b

Std Dev 4.4721 3.3166 16.8077

Std Err Mean 2.0000 1.4832 7.5166

Lower 95% 81.447 34.882 17.130

Figure 4: Onaway Analysis of Rooting (%) By Variant

136

Upper 95% 92.553 43.118 58.870

IBA for rooting influence of some varieties of pomegranate (Punica granatum L).

Figure 5: Different methods rooting

The average % of the root was 95 % and frequency variation between 40.5 % to 95 %. Variants averages each changing between each other in order to visible. V1 has high % of the roots than the order V2 and V3 and V2, V3 do not have differences between them. V 1 and V2 are prove statistical analisis, when V3 don’Ft have more prove from statistical analisis for some reason. Conclusion It is a tree that rotting light at costs as more advanced techniques and traditional ones in terms of the greenhouse. Only rigorously apply agricultural conditions

and

parameters

required

by

the

requirements of each method and the result will be that required. Each method has its own advantages and disaavantazh. The end result is that you planted in the field. Our country has all the conditions that add eta and innovation this tree, not only that consume destination and the natural resources, but also for economic purposes, market, ecosystems and the environment. References

1. Adakaveg J.E. and Forster H: Management of grey mold of pomegranate caused by Botrytis cinerea using two reduced – risk fungicides, fludioxomil and ferihexamid, Phytopathology 2002, 93:130.

4. Koppel K. and Chambers E.: Development and application of a lexicon to describe the flavor of pomegranate juice. J.Sensory Stud 2010, 25: 819 – 837. 5. Katz SR, Newman RA, Lansky EP. J.Med Food, 2007 : 10 (2); 213-217. 6. Kumar A, Dore J. Pomegranate ( Punica Granatum ) – Overview, 2012, Vo; 1 (4) 1218-1222. 7. Nowak R. Fatty acid composition in fruits of wild rose species. Acta Soc Botani. Cor Polo 2005, 74 (3): 229-235. 8. Ozgen M, Durgac C, Serce S and Kaya C: Chemical and antioxsidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey. Food Chem 2008, 111: 703-706. 9. Ozguven A.L, Yilmaz C and Keles D: Pomegranate biodiversity and horticultural management. Acta Hort 2012, 940:21-27. 10. Poyrazogly E, Gokmen V, and Artik N: Organik acids and phenolic compounds in pomegranate (Punica granatum L) grown in Turkey, Journal of Foods Composition and Analysis 2002, 15:567-575. 11. Koka T: Collection and Documentation of Pomegranate Germplasm in Albania. Acta Hort 2015, 1089: 375 – 378.

2. Basu A. and Penugonda K: Pomegranate juice a heart healthy fruit juiceNutr, Rev, . 2008, 67: 49-56.

12. Zhao X, Yuan Z Fang Y: Characterization and evaluation of major anthocyanins in pomegranate (Punica granatum L) peel of different cultivars and their development phases. Eur Food Res Technol, 2013, 236: 109-117.

3. Stover E. and Mercure E.W: The pomegranate: a new look at the fruit of paradise. Hort Science 2007, 42: 1088 – 1092.

13. Wang J.B, Yin W.Q and Yan C.W. Pomegranate dry not occurrence and integrated control technology. Shanxi Fruit 2006, 4: 24-25.

137

granatum L).

their irrigation. The data were processed statistically. ... Analysis of the data proved the main changes ... of the shoot (HL), have proven statistical homogeneity.

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