J4U1/8A16

Reg. No St. Joseph’s College of Arts & Science Autonomous) St. Joseph’s College Road, Cuddalore – 607001 BC405-INTERMEDIARY METABOLISM-II Time: 3 hrs

Max Marks: 75

SECTION-A (25X1=25) Answer ALL the questions: Choose the best answer (10X1=10) 1.

In humans, a dietary essential fatty acid is a) Palmitic acid b) Stearic acid c) Oleic acid d) Linoleic acid

2.

In mammals, the major fat in adipose tissues is a) Phospholipid b) Cholesterol d) Sphingolipids d) Triacylglycerol

3.

Long chain fatty acids are first activated to acyl-CoA in a) Cytosol b) Microsomes c) Nucleus d) Mitochondria

4.

A ketogenic amino acid is a) Valine b) Cysteine c) Leucine d) Threonine

5.

An important reaction for the synthesis of amino acid from carbohydrate intermediates is transamination which requires the cofactor: a) Thiamin b) Riboflavin c) Niacin d) Pyridoxal phosphate

6.

The main sites for oxidative deamination are a) Liver and kidney

b) Skin and pancreas

c) Intestine and mammary gland d) Lung and spleen 7.

The enzymes of urea synthesis are found in a) Mitochondria only

b) Cytosol only

c) Both mitochondria and cytosol d) Nucleus 8.

The number of ATP required for urea synthesis is a) 0

b) 1

c) 2

d) 3

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9.

In purine biosynthesis carbon atoms at 4 and 5 position and N at 7position are contributed by a) Glycine

b) Glutamine c) Alanine

d) Threonine

10. The key substance in the synthesis of purine, phosphoribosyl pyrophosphate is formed from a) D-ribose 5-phosphate b) 5-phospho β-D-ribosylamine c) D-ribose Say true or false

d) Deoxyribose (5X1=5)

11. The enzyme acyl-CoA synthase catalyses the conversion of a fatty acid to an active fatty acid in the presence of ATP. 12. The enzymes of β-oxidation are found in Mitochondria. 13. The enzyme carbamoyl phosphate synthetase requires Ca++. 14. The 2 nitrogen atoms in urea are contributed by Ammonia and aspartate. 15. Phosphorylation of adenosine to AMP is catalysed by adenosine kinase. Answer in one sentence (10X1=10) 16.

Lecithins are composed of _____

17.

What is the name of an enzyme required for the synthesis of ketone bodies as well as cholesterol?

18.

What are Ketone bodies and where it is synthesised?

19.

What are all the coenzymes required for the de novo synthesis of fatty acids?

20.

Which amino acid detoxicates benzoic acid to form hippuric acid?

21.

In which form ammonia is transported from muscles to liver mainly?

22.

Name the enzymes of urea cycle present in cytosol?

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23.

Which amino acid is not substrate for transamination?

24.

Expand N A D.

25.

Which enzyme of pyrimidine nucleotide biosynthesis sensitive to allosteric regulation?

SECTION – B (4X5=20) Answer any FOUR of the following: 26. Describe Urea cycle in detail. 27. What is beta oxidation? Explain. 28. Give an illustrated account on biosynthesis of phospholipids. 29. Write the degradative pathway of purine. 30. What is transamination? Explain with example. 31. Explain the types of detoxification. SECTION – C (3X10=30) Answer any THREE of the following: 32. Write an account on cholesterol metabolism. 33. Describe in detail about fatty acid synthesis. 34. Discuss pyrimidine biosynthesis. 35. Describe the mechanism of detoxification in detail. 36. Explain the Phase I & Phase II reaction.

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