2310651/650 Biochem Techniques : Spectrophotometry

Contents

1. Time schedule

page 1

2. Experimental Objectives

page 2

3. Experiment 1: Protein Determination

page 2

1.1 Quantitative Determination of Protein: Lowry Protein Assay

page 2

1.2 Qualitative Determination of Protein: Absorption Spectrum Analysis

page 3

4. Experiment 2: DNA Determination

page 6

2.1 Quantitative Determination of DNA

page 6

2.2 Qualitative Determination of DNA

page 6

5. Lecture note

P a g e |1

Time Schedule (22, 29 August, 5 September 2016) Mon August 22, 2016 13.00-16.00 Lecture: Room 503 (Dr. Suchart) - Introduction to Spectrophotometry Brief Lab: Room 503 (Dr. Suchart) - Spectrophotometric Assay Mon September 29, 2016 09.00-12.00 Lab: Room 504 (Dr. Suchart , Dr. Napa and Dr. Nuchanat) Experiment 1 : Protein Determination Assay 1.1 Quantitative Determination of Protein: Lowry Protein Assay 1.2 Qualitative Determination of Protein: Absorption Spectrum Analysis 13.00-16.00

Lab: Room 504 (Dr. Suchart , Dr. Napa and Dr. Nuchanat) Experiment 2 : DNA Determination Assay 2.1 Quantitative Determination of DNA: Ultraviolet Absorption at variable wavelength 2.2 Qualitative Determinations of DNA: Absorption Spectrum Analysis

Mon September 5, 2015 09.00-12.00 Lab Discussion: Room 521 (Dr. Suchart, Dr. Napa and Dr. Nuchanat) Lab results will be concluded and presented (in format of Microsoft Powerpoint) in classroom. Every group must hand in your presentation file (as a lab report) to Mr.Thara Junthathum by Tue September 6, 2016.

*** Quiz will be arranged at any time during lab experiments ***

Time Schedule-2016.pdf

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