Boyao Wang Research Assistant National Taiwan University No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan (R.O.C.) Depart of Chemistry Tel: 886-2-3366-8684 Email: [email protected]

Experience National Taiwan University, Research Assistant

2015-present

The Republic of China Army, Armored Cavalry, Corporal

2014-2015

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Lead a reconnaissance team during joint military exercise.

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Maintain, calibrate T91 assault rifle with night vision.

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Maintain radiation detector, toxic gas detector (mass spectrometer).

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Replace engine of M113 armored personnel carrier.

National Taiwan University, Senior Research Chemist 

Fix, set up and maintain Waters 996,600 HPLC system.

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Optimize epifluorescence imaging of mouse synapse with Ziess Z1.

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Establish synaptosome analysis protocol with FACSCantoII.

National University of Kaohsiung. Undergraduate Research Assistant 

Synthesized intermediate of chiral compound using nitrogen system.

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Handled moisture-sensitive compound using nitrogen blowing system.

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Using X-ray Diffraction Meter with silicon chip.

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Quantification antigen with ELISA.

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Total synthesis of Chiral Stationary Phases of column.

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Art work of cover accepted and published on Chemistry - A European Journal.

Technical Skills 

Biotechnology I.

Gel-electrophoresis (BIO-RAD Mini-PROTEAN Tetra System)

II.

UV-vis for Biology (BIOMATE 3S), (Hitachi/U3010)

III. Running ELISA based applications ( Multiskan EX Microplate Photometer) IV. Using and cleaning pipet (Gilson Pipetman® G) V. Quantified the content of biotin in protein using HABA assay VI. Modified protein with specific chemical group using crosslinking reagent VII. Conjugated protein to microsphere VIII. Using ImageJ to deconvolution image, and proceed human synapse imaging. IX. Labeling primary antibody with fluorescence (sigma mix-n-stain 405/488/633) 

Organic Chemistry Synthesis and Analyze

2012-2014

2010-2012

I.

Precede a moisture free synthesis (Grignard reaction)

II.

Gas Chromatography-FID (CH instrument/Model 600D Series)

III. High Performance Liquid Chromatography (Waters 996/600) IV. Nuclear Magnetic Resonance (Varian/Mercury plus 300 MHz) V. Fourier Transform Infrared Spectroscopy (Perkin Elmer/Spectrum RX 1) VI. Cyclic Voltammetry (CH instrument/Model 600D Series) VII. Fluorescence Spectroscopy VIII. Elemental analysis (2400 Series II CHNS/O System, PerkinElmer) IX. Powder XRD (Rigaku/multiflex 2kw) 

Computer Science I.

Operate System

ChemBio3D Ultra 13.0

Fedora 20

Mercury 2.3/2.4.6

Ubuntu 11.04

VISIONlite

II. Office software Mendeley Desktop 1.84 III. Graphics software Adobe Photoshop cs6 Adobe Illustrator cs6

UCSF Chimera 1.5.3 FlowJo 7.6.1 MestReNova Spartan 10 VI. Meth software

ImageJ 1.6.0_20

Wolfarm Methmatica 7

ZEN (blue edition)

MVSP

IV. Software Engineering Visual Studio Express 2008 Visual Basic V. Bio-Chemistry software

GraphPad Prism 6 SigmaPlot 12.0 PermutMatrixEN

The Merck Index v 13.1 ChemBioDraw Ultra 13.0

Education Master, Chemistry, National Taiwan University, June 2014 Bachelor of Science, Chemistry, National University of Kaohsiung, June 2011 General Diploma, National Chiayi senior high school, June 2008 General Diploma, Yu Shan public junior high school, June 2005

Non-Technical Skills 

Responsible for investigating and negotiating orders for equipment and supplies for laboratory use.

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Planned and organized social events.

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Experience in making posters for academic conferences

Publications 

Yu W. Chu, Bo Y. Wang, Huei S. Lin, Tai Y. Lin, Yuan J Hung, David A. Engebretson, Wonjae Lee,

James R. Carey Layer by layer assembly of biotinylated protein networks for signal amplification (2013) Chem. Comm. DOI: 10.1039/C2CC38233D 

Yu W. Chu, Bo Y. Wang, David A. Engebretson, James R. Carey Single step, rapid identification of pathogenic microorganisms in a culture bottle (2013) Analyst DOI: 10.1039/C3AN01175E

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H. C. Tai, B. Y. Wang, Serrano-Pozo A., M. P. Frosch, Spires-Jones, T. L.; Hyman, B. T. Frequent and symmetric deposition of misfolded tau oligomers within presynaptic and postsynaptic terminals in Alzheimer’s disease (2014) Acta Neuropath. Comm DOI: 10.1186/s40478-014-0146-2

Art works (cover) 

Dr. Seth E. Snyder, Bin-Syuan Huang, Yu W. Chu, Huei-Shian Lin, Prof. Dr. James R. Carey* DOI: 10.1002/chem.201290176

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Yu W. Chu , Bo Y. Wang , Huei-Shian Lin , Tai-Yen Lin , Yuan-Jen Hung , David A. Engebretson , Wonjae Lee and James R. Carey* DOI: 10.1039/C2CC38233D

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Yu W. Chu, Bo Y. Wang, David A. Engebretson, James R. Carey* (2013) Analyst DOI: 10.1039/C3AN01175E

Award 

Best paper award, 2014 Japan Taiwan Bilateral Conference on Biomedical and Plasmonic Imaging.

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優良壁報獎, 102 學年度研究生壁報展

Selected Presentations 

James R. Carey, Boshiuan Lin, Binsyuan Huang, Hueishian Lin, Yuwei Chu, Boyao Wang, Hungmiao Lin, Yuzih Chen, Yishan Wang, Menghuan Cheng, Haoju Chou, Jennshing Chen. Visual Detection of Pathogenic Microorganisms Using a Protein Conglomeration Method. (Aug 22-26, 2010) 240th American Chemical Society National Meeting, The Boston Convention and Exhibition Center, Boston, USA.

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James R. Carey, Boshiuan Lin, Binsyuan Huang, Hueishian Lin, Yuwei Chu, Boyao Wang, Hungmiao Lin, Yuzih Chen, Yishan Wang, Menghuan Cheng, Haoju Chou, Jennshing Chen. Signal Amplification by the Generation of Conglomerated Protein Networks. (Oct 22-25, 2010) 8th International Symposium for Chinese Inorganic Chemists, National Taiwan University, Taipei, Taiwan.

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James R. Carey, Hueishian Lin, Yuwei Chu, Boshiuan Lin, Binsyuan Huang, Boyao Wang, Hungmiao Lin, Yuzih Chen, Yishan Wang, Menghuan Cheng, Haoju Chou. Identification of Bacteria Using a Colorimetric Sensing Array. (Nov 2-5, 2010) 5th Asian Bioinorganic Chemistry Conference, The Ambassador Hotel, Kaohsiung, Taiwan.

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James R. Carey, Boshiuan Lin, Yuwei Chu, Hueishian Lin, Binsyuan Huang, Boyao Wang, Hungmiao Lin, Yuzih Chen, Yishan Wang, Menghuan Cheng, Haoju Chou. Using a Protein Conglomeration Method to Lead Visual Detection of Pathogenic Microorbanisms. (Nov 2-5, 2010) 5th Asian Bioinorganic Chemistry Conference, The Ambassador Hotel, Kaohsiung, Taiwan.

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Yu W. Chu, Bo Y. Wang, Bo S. Lin, Huei S. Lin, Bin S. Huang, James R. Carey. Signal amplification by

generation of protein networks. (Aug 28- Sept 1, 2011) 142th American Chemical Society National Meeting, The Colorado Convention Center, Denver, USA. 

James R. Carey, Boshiuan Lin, Binsyuan Huang, Hueishian Lin, Yuwei Chu, Boyao Wang, Yuzih Chen, Haoju Chou, Chenhao Chen, Shangjhih Jiang, Jisin Lin, Pinhsu Lin, Shengjie Lin, Yuanchen Hung, Taiyen Lin. Rapid identification of bacteria with a disposable chemical sensing array (Aug 28Sept 1, 2011) 142th American Chemical Society National Meeting, The Colorado Convention Center, Denver, USA.

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James R. Carey, Boshiuan Lin, Binsyuan Huang, Hueishian Lin, Yuwei Chu, Boyao Wang, Yuzih Chen, Haoju Chou, Chenhao Chen, Shangjhih Jiang, Jisin Lin, Pinhsu Lin, Shengjie Lin, Yuanchen Hung, Taiyen Lin. Assessing chiral self-recognition using chiral stationary phases (Aug 28- Sept 1, 2011) 142th American Chemical Society National Meeting, The Colorado Convention Center, Denver, USA.

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Yuwei Chu, Boshiuan Lin, Hueishian Lin, Boyao Wang, Yuanjen Hung, Taiyen Lin, Chenhao Cgen, Shengjie Lin, Haoju Chou, James R. Carey. Signal amplification via a protein crosslinked network (Sept 5-8, 2012) 國立高雄大學應用化學系 100 學年度專題研究壁報論文競賽, National University of Kaohsiung, Kaohsiung, Taiwan.

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Huei-Shian Lin, Bo-Shiuan Lin, Yu-Wei Chu, Bin-Syuan Huang, Bo-Yao Wang, James R. Carey. Detection of Pathogenic Microorganisms Using Streptavidin Coated Microspheres. (Aug 28- Sept 1, 2011) 142th American Chemical Society National Meeting, The Colorado Convention Center, Denver, USA.

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Bo Y. Wang, Alberto Serrano-Pozo, Matthew P. Frosch, Tara Spires-Jones, Hwan-Ching Tai, Bradley T. Hyman. Direct visualization of misfolded tau oligomers at pre- and post-synaptic terminals in Alzheimer’s disease. (Fab 25-26) 2014 Japan Taiwan Bilateral Conference on Biomedical and Plasmonic Imaging, National Taiwan University, Taipei, Taiwan.

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Bo Y. Wang, Huei-Ting Chen, Gina Renschler, Alberto Serrano-Pozo, Matthew P. Frosch, Tara SpiresJones, Bradley T. Hyman, Hwan-Ching Tai. Analysis of synaptic tau protein localization by immunofluorescence microscopy and flow cytometry. (May 7) 102 學年度研究生壁報展