Q7/20R/11-14 Reg. No

St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001 EPMB705S - METHODS IN BIOLOGY

Time : 3 hrs

Max Marks :75 SECTION – A (15X1=15) Answer any FIVE Questions

Choose the correct answer 1. Name the spectroscopy working with Beer-Lambert principle. a. ESR b. NMR c. UV/Visible d. Centrifuge 2. Identify the biological chromophore molecule. a. Nucleic acid b. Carbohydrate c. Vitamin 3. Fluorescent probe is a. Ethidium bromide c. Carbon dioxide

d. Lipid

b. Calcium amide d. Magnesium chloride

4. NMR is---a. Numeric Magnetic Resonance b. Nucleus Magnetic Resonance c. Nuclear Magnetic Resonance d. Nano Magnetic Resonance 5. What is the precipitant of protein in X-ray crystallography? a. Polyethylene glycol b. Alcohol c. Sodium d. Potassium 6. What is SPR? a. Sub plasma resonance b. Surface plasma resonance c. Simple plasma resonance d. Super plasma resonance 7. Radioisotopes liberate energy in the form of a. Alpha b. Beta c. Gamma

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d. All

Q7/20R/11-14 8. Radioisotopes found in nature are a. Uranium b. Plutonium c. Radium

d. All

9. The half-life of 42K isotope is -------. a. 12.40 hours b.14.20 days c. 2.26 years

d. 2.26 days

10. Tungsten microelectrodes are related to ----------. a. Patch clamp recording b. Single neuron recording c. Multiple ion channels d. Reverse ion channels 11. Patch-clamp technique allows the investigation of --------------. a. Double ion channels b. Single ion channels c. Multiple ion channels d. Reverse ion channels 12. PET is -------. a. Positive emission tomography b. Positron emission tomography c. Post emission tomography d. Potassium electronic testing 13. Events are called -------------- behaviour. a. Instantaneous b. Appreciable c. Abnormal

d. Altered

14. States are ---------- behaviour. a. Instantaneous b. Appreciable

d. Altered

c. Abnormal

15. counting every member of a population is called a. Complete count b. Total count c. Plate count d. Standard count SECTION – B (5X12=60) Answer any FIVE Questions 16. What do you know about NMR? Explain the analysis of biomolecules using NMR with illustrations. 17. How the UV/visible spectroscopy used to analyse the biomolecules? Explain with suitable diagrams.

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Q7/20R/11-14 18. Explain the NMR application in biologically important molecules. 19. Give the flow chart of X- ray crystallographic method and explain in detail. 20. What are the safety guidelines of the radioisotopes? Explain in detail. 21. What is ECG? Explain the principles of ECG with diagram. 22. Distinguish between MRI and CAT with illustrations. 23. Explain in detail the methods of estimating population density.

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