USO0PP23321P2

(12) United States Plant Patent Deuter (54)

MISCANTHUS PLANT NAMED ‘MBS 0006’

(50)

Latin Name: Miscanthus Varietal Denomination: MBS 0006, a.k.a 99m0029,

Martin Deuter, Wanzleben (DE)

(51)

Int. Cl. A01H 5/00

(52)

U.S. Cl. ..................................................... .. Plt./384

(58)

Field of Classi?cation Search .................. .. Plt./384

Notice:

(56)

Subject to any disclaimer, the term of this patent is extended or adjusted under 35

PPl3,008 PPl5,l93 PPl6,l97 PPl8,l6l PP22,033 PP22,047 PP22,l27

(21) Appl. No.: 13/385,459 Feb. 22, 2012 Related U.S. Application Data

(60)

References Cited U.S. PATENT DOCUMENTS

U.S.C. 154(b) by 0 days.

(22) Filed:

(2006.01)

See application ?le for complete search history.

(73) Assignee: Mendel Biotechnology, Inc., Hayward, CA (U S) (*)

Jan. 8, 2013

(60) Provisional application No. 61/050,162, ?led on May 2, 2008.

99m0006 or Flamingo Rest

(75) Inventor:

US PP23,321 P2

(10) Patent N0.: (45) Date of Patent:

P2 P2 P2 P2 P2 P2 P2

9/2002 9/2004 l/2006 10/2007 7/20ll 7/20ll 9/20ll

Walsh Smith et a1. Weiskott Probst Deuter Deuter Deuter

Primary Examiner * Susan McCormick Ewoldt

Division of application No. 13/067,951, ?led on Jul. 8,

(74) Attorney, Agent, or Firm * Cooley LLP; Jeffrey M.

2011, now abandoned, which is a continuation of

Libby; Yifan Mao

application No. 12/584,496, ?led on Sep. 4, 2009, now Pat. No. Plant 22,127, which is a continuation-in-part

(57)

of application No. 12/387,444, ?led on May 1, 2009,

A new and distinct cultivar ofMiscanthus named ‘MBS 0006 ’

ABSTRACT

now Pat. No. Plant 22,033, and a continuation-in-part

generally characterized by its rigorous growth rate, high bio

of application No. 12/387,429, ?led on May 1, 2009,

mass yield and high tiller density, and/or improved cold tol

now Pat. No. Plant 22,047, and a continuation-in-part

erance.

of application No. 12/387,437, ?led on May 1, 2009, 5 Drawing Sheets

now abandoned.

1

2

Latin name of genus: Miscanthus.

isolated poly-cross population (‘114SW’) at Klein Wanzle

Varietal denomination: ‘MBS 0006’, aka ‘99m0029’,

ben produced seed on various highperforming biomass types.

‘99m0006’ or ‘Flamingo Rest’.

Seed collected from this population was named ‘95s0043’ and was again planted at Klein Wanzlchlen for recurrent selection and advancement of improved biomass clonal lines. From this population an individual plant was selected for

BACKGROUND The present disclosure relates to a new and distinct cultivar

vigorous growth, high biomass and cold tolerance potential.

of hybrid Miscanthus originated as a polycross of Miscanthus sinensis with other Miscanthus species and will be referred to hereafter by its cultivar name ‘MBS 0006’. ‘MBS 0006’ represents a new cultivar of Miscanthus species, a perennial grass which is grown for biomass production, landscape use, ornamental value, and cold tolerance. Seeds of an open-pollinated Miscanthus sinensis plant in a natural Miscanthus habitat at Shirakawa-Gun, Honshu, Japan

The accession was named ‘99m0029’, a.k.a. ‘MBS 0006’, ‘99m0006’, or ‘Flamingo Rest’.

The plant was asexually reproduced from sterile rhizome buds in Klein-Wanzleben, Germany by the inventor. The shoot material was propagated on rooting media and the

rooted plantlets were planted into pots in the greenhouse. The plants were planted into the ?eld after one cold period. The characteristics of this cultivar have been determined to be stable and are reproduced true to type in successive genera

were planted and screened in a cultivated selection ?eld at

Klein-Wanzleben, Germany. The selection ?eld was utilized for seed increase of superior genotypes derived from the original plant selection. Additional recurrent selection in the newly derived, ?nite population was utilized to derive addi tional selection pressure for line improvement.

tions. ‘MBS 0006’ was transferred to US via APHIS quaran

tine inspection regulations and was established for perfor mance trials in the US. ‘MBS 0006’ performance trials 20

Miscanthus sinensis is self incompatible. Presumably, the Miscanthus sinensis plant in the natural habitat in Japan became pollinated by other Miscanthus plants, hence it could be regarded as a polycross of M sinensis plants. Therefore, its continued lineage/pedigree was derived from seed collected on this plant selection. The open pollinated seed from the population plant selection was a bulk collection of half-sib

lines (genotypes) from that mother plant, and were planted for advanced recurrent selection in an organized Miscanthus sin

ensis breeding nursery at Klein Wanzleben, Germany. The

include biomass yield and trait characteristic observations. ‘MBS 0006’ has been evaluated in mutli-entry, replicated performance trials. These trials ranged across three diverse US locations targeted as US biomass growing regions of the

country. 25

SUMMARY OF THE INVENTION The new cultivar ‘MBS 0006’ has not been observed under

all possible environmental conditions. The phenotype may vary somewhat with variations in temperature, day-length,

US PP23,321 P2 3

4

light intensity, soil types, and Water and fertility levels With out, however, any signi?cant variance in genotype.

tral and southern regions of the US and Klein WanZleben,

Germany. The folloWing observations, measurements, and

The following traits have been repeatedly observed and are determined the basic characteristics of ‘MBS 0006’, Which in

Germany, When groWn in the ?eld. All observations Were

combination distinguish this Miscanlhus sinensis hybrid

recorded during the plant’s dormant season (April). The color

from the knoWn Miscanlhusxgiganleus and other ornamental

determination is in accordance With The 1995 R.H.S. Colour

M sinensis forms. Plants for the botanical measurements in

the present application are tWo and three-year-old plants.

Chart of The Royal Horticultural Society, London, England, except Where general color terms of ordinary dictionary sig

These plants Would be considered as mature plants.

ni?cance are used. Plants for the botanical measurements in

comparison describe this plant as groWn at Klein-WanZleben,

1. Vigorous groWth

the present application are tWo and three-year-old plants.

2. Plant height: approximately 3 m at or above 35 deg N latitude and about 2.5 m at or beloW 35 deg Northern latitude. 3. Green leaves, no colored stripes are present, but green leaves have a creamy White leaf mid rib characteristic of M sinensis biomass genotypes.

These plants Would be considered as mature plants. Additional and most relevant performance data included in this application includes observations, measurements and comparisons describing this plant that Were recorded in ?elds

groWn at Tilton, Ga., Schocho, Ky., and Lafayette, Ind. Reported data exempli?es this genotype’s ability to perform

4. High biomass yield compared to several Miscanlhus

as a high biomass producing perennial crop for commercial

cultivars.

markets. Therefore, beyond descriptive data, yield traits are presented and compared to commercial and pre-commercial

5. High culm thickness. 6. Excellent area of adaptation for both Warm and cold

climates of biomass groWing regions.

20

‘MBS 0006’ can be distinguished from the Miscanlhus

derived from selected polycrossed M sinensis plants. ‘MBS 0006’ is a diploid (2x) With chromosome comple

cultivars Strictus (not patented), Super Stripe (U.S. Plant Pat. No. 18,161), Gold Bar (U.S. Plant Pat. No. 15,193), Little Zebra (U .S. Plant Pat. No. 13,008) and Mysterious Maiden (U.S. Plant Pat. No. 16,197) in that ‘MBS 0006’ has no stripes

cultivars associated With this market. Botanical classi?cation: ‘MBS 0006’ is a hybrid genotype

25

ment of 38. Common name: Maiden grass.

In side by side comparisons conducted in Klein-WanZle

Parentage: Polycross of M sinensis originated from Japan and other unknoWn Miscanlhus species originated from

ben, Germany, ‘MBS 0006’ is more vigorous than other oma mental M sinensis. ‘MBS 0006’ has taller culms but demon strates less lodging; hence it has stronger culms. ‘MBS 0006’

recombined in a neW poly cross of Which the parental plant selection is indicated in FIG. 1.

or colored bands on its leaves.

Japan. Plant selections from that original population Were 30

culms are also very straight and medium upright in appear

The plant ?oWers and produces fertile seeds. ‘MBS 0006’ develops in?orescences and viable seeds under optimal groW ing conditions. HoWever, ‘MBS 0006’ is 98% self incompat ible. Therefore, it requires cross pollination With different Miscanlhus genotypes to produce viable seeds. The plant can be propagated by rhiZomes or from meristem in tissue culture or adventitious root and shoot development under ideal veg

summer in the southern US and late summer to mid

fall in central US. It maintains a relatively indetermi 35

40 deg North bloom during early to late fall. Blooms are retained for a period of time, but blooming com 40

BRIEF DESCRIPTION OF THE DRAWINGS

is signi?cantly thicker in this yield trial measurement (Tables

45

50

(north central), Germany and Ontario, Canada. It has

CulZureiBest in sandy loam, Well-drained soil, higher yields at higher soil fertility. Diseases and peslsiln United States ‘MBS 0006’ has shoWn excellent resistance to Miscanlhus blight When

compared to the public check variety M giganleus cv. Illinois. Root descriplioniFibrous, Well branched and dense.

55

Fast-developing creeping rhiZomes With shoots aris ing about 1-2 feet from base of the culms depending on ?eld plant density. GroWth and propagation: PropagalioniBy culm division, in vitro culture, from

60

rhiZomes or meristem. Seed can be harvested and

FIGS. 6, 7, and 8 illustrate mid-season plant and panicle development at Tifton, Ga. in July.

propagated if alloWed to pollinate With other Miscanl hus genotypes/parents for crosses or seed production.

Growth raleiVigorous.

‘MBS 0006’ has not been observed under all possible envi

ronmental conditions, and the phenotype may vary signi? cantly With variations in environment. It has been observed under several environmental conditions, including mid, cen

HardinessiProductive groWth in Klein-WanZleben very good drought and heat resistant in southern regions beloW 30 deg N latitude.

1-4).

DETAILED BOTANICAL DESCRIPTION OF THE PLANT

pletely shuts doWn after frost. Plant habiLiHerbaceous, tuft forming, biomass grass With upright culms, With 15-17 leaves per culm. Height and spreadiTop leaf height about 2.9-3.0 meters.

appearance and distinct characteristics of the neW Miscanlhus cultivar ‘MBS 0006’.

The photograph in FIGS. 1 and 2 illustrate the overall groWth habit and appearance of ‘MBS 0006’ at Winter dor mancy and in the summer, respectively, in Tifton, USA. FIG. 3 illustrates midseason green color, glabrous leaf texture and the White mid-rib characteristic to ‘MBS 0006’. FIGS. 4 and 5 illustrate culm/ cane thickness of midseason and dormant ‘MBS 0006’. The culm Width of ‘MBS 0006’ is a unique characteristic among the comparison group in that it

nate groWth habit regarding continuous culm and panicle production until early frost in the fall. Plants groWn in northern locations close to and Well above

etative propagation environments.

The accompanying color photographs illustrate the overall

General description: Blooming periodi‘MBS 0006’ may bloom in mid

ance When compared to other sinensis genotypes. It shoWs excellent Winter survival at all northern and southern loca tions tested.

65

Culm (stem) description: (Plant ages for data beloW are from tWo and three-year-old plants. They Would be considered mature plants.)

US PP23,32l P2 6

5 GeneraliCylindrical, pithy, reed-like, erect, sheathed.

GynoeciumiStigma color is purple 186C, about 4 mm

Culm thickness is signi?cant for this M sinenesis

in length and about 0.5 mm in Width.

CaryopsisiProduces fertile seeds.

genotype. Culm aspecLiRigid and held erect, none are cascading. Culm lengZlLiAverage about 2.5-3 m. Culm diameZeniAverage about 6.7-7.8 mm. Plant widZlLiThe average plant Width is dependent on ?eld planting density, but as a stand alone plant its

REPRODUCTION

‘MBS 0006’ has a small elliptical seed, Which is a heavy anemochore With an average seed Weight of about 0.92 mg, a

chamaephyte (buds permanently above ground) life form,

Width can be about 2-3 m.

Plant heighLiAverage about 2.7-3.0 meters.

With an annual seed production of 0-2400 seeds per plant

Culm color (dormant season).iYelloWish (2D), loWer

depending upon pollen source availability. Healthy seed is clear amber to dark broWn (RHS code 166B).

internodes partly reddish (53A), and vary With inten sity depending on locations and groWing conditions.

YIELD

The rate on Which the culm color changes is depen dent on location and groWing conditions.

The average yield of ‘MBS 0006’ compared to appropriate

Compressed circumferenceiAbout 120 cm on a 2 year

old plant, fully compressed With standard compres

commercial and pre-commercial check cultivars are summa

sion belt.

riZed in Tables 1 through 4.

Culm surface ZexZure.4Culm is glabrous. lnlernode lengZlLiVaries by location on plant and groWing environment (about 5 cm to about 15 cm). LiguleiMembranous, about 5 mm (M giganleus cv

Variety comparisons Were selected for this patent applica 20

Illinois is 2.5-3 mm), color reddish 145B, border 58A, longest hair is about 4 mm (M giganleus cv Illinois is 1 mm), encircles the entire culm, inner surface is

glabrous, glabrous outer surface, and side.

tion based on commercial and pre-commercial genotypes

With similar and competitive yields levels and uniqueness to sister lines of similar family. Comparison to M sinensis cul tivars that are commercially available are only used in refer ence to morphological phenotype because available cultivars are not biomass types of varieties.

25

Although ‘MBS 0006’ Was not the highest biomass yielder

Cold hardinessiSurvives in Germany and Canada.

across or Within locations it Was alWays in the top 10% of the

CulZureiBest in sandy loam, Well-drained soil, higher

full trial analysis. In the truncated analysis Which included entries of relevance for biomass comparisons ‘MBS 0006’ Was competitive With the best pre-commercial MBI cultivars, and out-performed all of the most competitive sinensis lines. A key biomass trait is culm thickness and ‘MBS 0006’ has a signi?cantly thicker culm than the other entries in the test. This trait is important in that it can bring value to biomass yield parameters in Miscanlhus as Well as to its utility in

yields in Warmer climates and higher soil fertility.

Foliage description: Leaf shapeiLinear.

30

Leaf margin .4Green 140A.

Leaf divisioniSimple. Leaf apeyaiAcuminate. LeafaspecLiEmerging leaves are erect, blades are con

vex, leaf angle of younger leaves is 50°, leaf angle of older leaves is 5°, color code 154C. Leaf length and widZlLiAbout 2.5 cm in Width and

35

For Tables 1 to 4, all traits presented Were measured at and

harvest during Winter dormancy groWth stage. De?nitions for

about 50 cm in length.

Leaf baseiSheathed. Leaf aZZachmenLiSheathed.

Leaf arrangemenLiAlternate.

the terms and abbreviations are:

l. Biomass dt/ac: Average post senescence, and dormancy 40

midrib, green color code is 140A.

3. Culm Dia: Average culm diameter measured in mm

FloWer description:

across mid section of culm

General descripZion.4Compact, fan-shaped panicle 45

slender, silky aggregate racemes. 50

Laslingness of in?orescenceiFor emergence until panicle dehiscence: about 2.5 months.

FragranceiNone detected. Panicle sizeiAverage of about 12-15 cm in length and about 8 cm in Width at seed maturation stage. Panicle coloriVaries from 154A to 2D at maturity. The

to trial entries.

60

65

7. Lodge: l-5, Where 1 is the least observed plant/culm lodging from base of the plant after fall and Winter dormancy relative to trial entries, and 5 is the highest observed plant/culm lodging from base of the plant after fall and Winter dormancy. A rating of 3 represents aver

55

Spikelel descripZioniSpikelets in pairs. Spikelel coloril 54B.

Width (excluding hairs). Spikelel hairsiAbout 14 mm in length, 158C in color. Awn sizeiAbout 2 mm.

Reproductive organ description: AndroeciumiAnthers about 5 mm in length, and about 0.5 mm in Width, reddish purple in color, 187A.

observed relative to trial entries. A rating of 3 represents average and medium culm density relative to trial entries. 5. Biomass Rating: l-5, Where 1 is the least observed biomass appearance relative to trial entries, and 5 is the highest biomass observed relative to trial entries. A rat ing of 3 represents average and medium biomass relative 6. Leaf Retention: l-5, Where 1 is the least observed leaves retained on the plant after fall and Winter dormancy relative to trial entries, and 5 is the highest observed leaves retained on the plant after fall and Winter dor mancy relative to trial entries. A rating of 3 represents average and medium leaf retention relative to trial entries.

intensity of these color grades depends on location and groWing conditions. Spikelel sizeiAbout 8 mm in length and about 2 mm in

4. Culm Density Rating: l-5, Where 1 refers to the least culm density visually observed relative to the trial

entries, and 5 is the highest culm density visually

October in the north. It is composed of numerous Angle of racemeiVaries With age of panicle i see FIGS. 7 and 8.

harvest measured in dry tons per acre.

2. PH: Average total plant height measured in cm from soil

surface to base of panicle (5 culm subsample per plot).

Leaf color (during growing season).iGreen, White

terminating from each culm in mid to late July through September in the south and September to late

breeding programs.

age and medium lodging relative to trial entries.

US PP23,321 P2 11

12

TABLE 4-c0ntinued

TABLE 4-c0ntinued

Yield summary for ‘MBS 0006’ (‘99m0006’) performance on

Yield summary for ‘MBS 0006’ (‘99m0006’) performance on

two-year established Miscanlhus ?eld plots, located at Schocho, KY,

two-year established Miscanlhus ?eld plots, located at Schocho, KY,

growing season.

growing season.

APHIS Miscanlhus spp. Diversity Trial: 2nd Year Mean Yield Data at Schochoh, KY

5

APHIS Miscanlhus spp. Diversity Trial: 2nd Year Mean Yield Data at Schochoh, KY

Schocho, KY: March, 2nd Yr Harvest Yield Traits

5 10 6 4 3 7

10

Culm

Biomass

Leaf

99m0005 99m0003 99m0044 Mean

Density

Rating

Retention

LSD(.05)

Ratings 1-5 Material

Entry Name

Species

99m0006 Msi:Mis sinensis MEX-005 Msa//Msi 99m0029 Msi :Mis sinensis

3 4 4 3 3 2 4.0

Msi:Mis sinensis Msi:Mis sinensis Msi :Mis sinensis

CVErr

11

MBX-006 Msa//Msi MBX-004 Msa//Msi

1 N?gm .

.

5 4

4.3 4.3

5 5

4

4'7

5

4 5 4

4.0 4.0 3 .0 3.9

4 4 2 4.0

1.0

1.4

1.0

14.7

21.9

13.1

15

MxgIMiS X g‘game?”

4.3 3 .7 3 .6

The invention claimed is:

2

Illinois

Mxg:Mzs X

gigameus

4

35

5

8

Lake Erie

Msa//Msi

5

3.7

5

1. A neW and distinct cultivar of Miscanlhus plant named ‘

20

,

.

.

.

MBS 0006 substantlally as hereln shown and described.

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Miscanthus plant named 'MBS 0006'

Feb 22, 2012 - 2011, now abandoned, which is a continuation of application No. 12/584,496, ?led on Sep. 4, 2009, now. Pat. No. Plant 22,127, which is a ...

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