Novel antiviral compound K18 effective against HSV-1 Bhupesh K Prusty1, Lina Niu2, Kirk Kimmerling3, Allen D Johnston4, Franklin Tay2, 3,5 1 3,6 Gerhard RF Krueger , Thomas Rudel , Dharam Ablashi
Lehrstuhl für Mikrobiologie, Julius-Maximilians-Universität Würzburg, Germany. 2Department of Endodontics, College of Dental Medicine, Georgia Regents University, Augusta, USA. 3KHG fiteBac Technology, Marietta, USA. 4DM Healthcare Products, Inc., San Diego, USA. 5 Department of Pathology and Laboratory Medicine, UT-Houston Medical School, Houston, USA. 6HHV-6 Foundation, Santa Barbara, USA. 1
Relative HSV-1 DNA
Results 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0
K18 in Ethanol
0μM
2μM
K18 in DMSO
1μM
0,5μM
Figure 1: K18 inhibits HSV-1 infection in Vero Cells. Vero cells were infected with HSV-1 (MOI 0.5) in presence of different concentrations of K18 dissolved either in DMSO or Ethanol. Total DNA was extracted after 24hrs of viral infection and was used for qPCR to determine viral DNA amount. * HSV-1 strain F (ATCC VR-733)
Conclusions 1. K18 downregulates HSV-1 infection at certain concentrations without cell toxicity. However, has no effect at higher concentrations. 2. K18 has similar property of inducing HSV-1 infection-mediated host cell death like K21, possibly by inducing senescence after 48-72 hrs of viral infection.
