Novel antiviral compound K21 effective against HSV-1 Bhupesh K Prusty1, Lina Niu2, Kirk Kimmerling3, Allen D Johnston4, Franklin Tay2, 3,5 1 3,6 Gerhard RF Krueger , Thomas Rudel , Dharam Ablashi

Lehrstuhl für Mikrobiologie, Julius-Maximilians-Universität Würzburg, Germany. 2Department of Endodontics, College of Dental Medicine, Georgia Regents University, Augusta, USA. 3KHG fiteBac Technology, Marietta, USA. 4DM Healthcare Products, Inc., San Diego, USA. 5 Department of Pathology and Laboratory Medicine, UT-Houston Medical School, Houston, USA. 6HHV-6 Foundation, Santa Barbara, USA. 1

Results -Trypsin

Relative HSV-1 DNA

1.2

+Trypsin

1.0 0.8 0.6 0.4 0.2 0

0μM

1.3μM

1μM

0.5μM

0.33μM

0.25μM

Figure 1: K21 inhibits HSV-1 infection in Vero Cells. HSV-1 viral particles were incubated in presence of different concentrations of K21 for 3 minutes at room temperature. As K21 was dissolved in Acetone and water, viral particles were also incubated for 3 minutes with highest amount of acetone containing water in the absence of any K21 (0μM). After 3 minutes of exposure, viral particles were allowed to adhere and enter the cell for 30 minutes. One part of the cells were trypsinized (+ Trypsin) and washed thoroughly to remove viral particles that were bound to the outer cell surface but not entered into the cell. Other half was not trypsinized (- Trypsin) but washed thoroghly to remove unbound viral particles. Total DNA was extracted and was used for qPCR to determine viral DNA amount. CC50 = 8.5μM. * HSV-1 strain F (ATCC VR-733)

Conclusions 1. K21 inhibits viral infection when viral particles are exposed to K21 for 3 minutes.