QUIZ NO: - 01 QP- A

QP-B

1. Define the Following: i) Retention Time ii) Internal Standard

1. Define the Following: i) Resolution ii) Gradient Elution

2. Justify the Following: i) Polarity of compound which would be easily eluted from Reverse Phase Chromatography column

2. Justify the Following: i) Isolation and purification of synthetic chemical mixture were performed by Preparative chromatography.

3. Explain in detail about:i) Van- Deemter Equation ii) Internal Standard Method

3. Explain in detail about:i) Area Normalization Method ii) HETP

4. Calculate A chromatogram of a mixture of A & B provided from the following data of HPLC, where column length was found to be 25 cm

4. Calculate A chromatogram of a mixture of A & B provided from the following data of HPLC, where column length was found to be 15 cm

NonRetained A B Calculate

Retention Time 2.1

Peak width

16.40 17.60

1.11 1.21

a) Capacity Factor of A b) HETP for B

NonRetained A B Calculate

Retention Time

Peak width

7.52 10.14

0.51 0.94

a) Number of Theoretical plates for A b) Resolution between A & B