IV B.Tech I Semester Examinations,November 2010 DOWNSTREAM PROCESSING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
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1. Describe the mode of operation of super critical fluid extraction .Give a neat sketch of the block diagram. What are the advantages of super critical fluid in the extraction operation? [16] 2. Discuss the steps involved in the product isolation and purification of penicillin production. [16] [16]
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3. Describe electrophoresis separation methods.
4. Write in detail about the precipitation methods based on solvent property modification. [16]
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5. Write about Bioaffinity chromatography and how to reduce non specific interactions in affinity chromatography. [16] 6. (a) What are protein inclusion bodies? How are they converted to native proteins? (b) What is cell permeabilization? What is its use?
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7. Discuss membrane filtration with respect to different types of membranes and different configurations of membrane separation equipment. [16]
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8. Discuss about the Important steps involved in downstream processing for butanol production. [16]
1
Code No: R05412304
R05
Set No. 4
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IV B.Tech I Semester Examinations,November 2010 DOWNSTREAM PROCESSING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. (a) What are protein inclusion bodies? How are they converted to native proteins? [16]
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(b) What is cell permeabilization? What is its use?
2. Describe the mode of operation of super critical fluid extraction .Give a neat sketch of the block diagram. What are the advantages of super critical fluid in the extraction operation? [16] [16]
or
3. Describe electrophoresis separation methods.
4. Write about Bioaffinity chromatography and how to reduce non specific interactions in affinity chromatography. [16]
uW
5. Discuss membrane filtration with respect to different types of membranes and different configurations of membrane separation equipment. [16] 6. Discuss the steps involved in the product isolation and purification of penicillin production. [16] 7. Write in detail about the precipitation methods based on solvent property modification. [16]
?????
Aj
nt
8. Discuss about the Important steps involved in downstream processing for butanol production. [16]
2
Code No: R05412304
R05
Set No. 1
in
IV B.Tech I Semester Examinations,November 2010 DOWNSTREAM PROCESSING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Discuss about the Important steps involved in downstream processing for butanol production. [16]
ld .
2. (a) What are protein inclusion bodies? How are they converted to native proteins? (b) What is cell permeabilization? What is its use? 3. Describe electrophoresis separation methods.
[16]
[16]
or
4. Describe the mode of operation of super critical fluid extraction .Give a neat sketch of the block diagram. What are the advantages of super critical fluid in the extraction operation? [16]
uW
5. Discuss membrane filtration with respect to different types of membranes and different configurations of membrane separation equipment. [16] 6. Discuss the steps involved in the product isolation and purification of penicillin production. [16] 7. Write about Bioaffinity chromatography and how to reduce non specific interactions in affinity chromatography. [16]
?????
Aj
nt
8. Write in detail about the precipitation methods based on solvent property modification. [16]
3
Code No: R05412304
R05
Set No. 3
in
IV B.Tech I Semester Examinations,November 2010 DOWNSTREAM PROCESSING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Discuss membrane filtration with respect to different types of membranes and different configurations of membrane separation equipment. [16]
ld .
2. Write in detail about the precipitation methods based on solvent property modification. [16] 3. Write about Bioaffinity chromatography and how to reduce non specific interactions in affinity chromatography. [16]
or
4. Discuss the steps involved in the product isolation and purification of penicillin production. [16] 5. Discuss about the Important steps involved in downstream processing for butanol production. [16]
uW
6. (a) What are protein inclusion bodies? How are they converted to native proteins? (b) What is cell permeabilization? What is its use? 7. Describe electrophoresis separation methods.
[16] [16]
?????
Aj
nt
8. Describe the mode of operation of super critical fluid extraction .Give a neat sketch of the block diagram. What are the advantages of super critical fluid in the extraction operation? [16]
[16] · 4. What are channel,circuit and half circuit in the context of satellite telemetry sys- .... 3. Describe the conditions for total internal reflection within a fibre. [16].
(b) Describe a DC Servo Motor with a neat sketch. [8+8] · 4. (a) What is a timing belt? Discuss the use of Timing belts in power transmission? (b) Discuss various ...
(b) Consider any two networks of your choice which support Circuit switching or ... (b) Which is more efficient, circuit switching or packet switching? Why?
(a) What is the difference between logical I/O and device I/O? (b) Explain feed back scheduling policy. [8+8] · 2. (a) How can logical address space be ...
kerosene, and fuel oil must be limited as also shown in this table. There are no ... equipment is a function of x, the size (horsepower ) of the motor, namely.
(b) What is meant by displacement function? (c) Give the importance of Pascal Triangle. [4+6+6] · 3. Determine the displacements at nodes and the stresses in ...
The horizontal distance between u/s face and center line of drain holes is 8 m. ... (c) Cut-off trench ... Bed width of parent channel U/S / D/S = 45 m/40 m. [16].
Also draw the relevant circuit diagram. (b) Draw the resistive model of a CMOS inverter and explain its behavior for. LOW and HIGH outputs. [8+8]. 8. (a) It is necessary to identify the position of mechanical disk, when rotates with a step of 450. Gi
4. Give the syntax for 'awk'. Discuss about the selection criteria in awk. How to · embed action in to this command. Explain with examples. [16] · 5. Write about different Unix locking techniques. [16] · 6. (a) What are the differences between shell
of Rs. 1,500 would be deposited in this fund under an ordinary annuity plan. All · money in the fund can be .... (b) Discounted cash flow · (c) Net present worth ... would last for 10 years. A worn-out storage tank of capacity equivalent to the new.
R05. Set No. 2. III B.Tech I Semester Examinations,May 2011 · WEB TECHNOLOGIES · Information Technology ... that describes your college. The paragraph ...
(b) What is the necessity of heat treatment after carburizing? Explain. On what ... (b) What is the difference in heat treatment between 18% Ni and the 25% Nickel.
is subjected to a load of 200 kN, find the stresses developed in the two materials. ... Compare the values of max. and minimum hoop stresses for a cast steel ...
The cores of two identical transformers A and B carry alternating fluxes whose instantaneous values are (0.01 sin 314t)W and 0.012 (1.1 sin 628t+0.1 sin 1884t).
(a) Explain live node, E-node and dead node with an example. (b) Explain the method of reduction to solve TSP problem using Branch and · Bound. [8+8] · 3. (a) Prove that Prim's algorithm finds a minimum spanning tree. (b) Explain the single source sh
at 0.8 p.f. lagging and 15A at 0.6 p.f. lagging respectively, both expressed ... (b) A single-phase motor takes a current of 10 amps at a p.f. of 0.707 lagging.
If the tensile stress is not to exceed 17MPa, find the safe. u.d.l. which the beam can carry. Also find maximum compressive stress. Draw the sketch showing the ...
