1. (a) What do you mean by simple media & complex media?
[8+8]
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(b) Write the advantages of complex media over simple media.
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II B.Tech II Semester Examinations,December 2010 BIOPROCESS ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
2. What are the applications of aerobic fermentation processes in biotechnological industries? [16] 3. Explain growth energetics using steady state continuous culture of S.cereviseae. [16]
or
4. How mass transfer plays an important role in the design of a fermenter?
[16]
5. Describe solid-state fermentation. What is a Koji process and why is it used in major industrial production of enzymes? [16]
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6. Derive the expression of specific growth rate for multiple substrate utilization. Specify what type of substrate inhibition it is? [16]
7. Write short notes on the following:
(a) Precursors and inducers in medium formulation (b) Structured growth model of microorganisms (c) Oxygen and Carbon dioxide exhaust gas analyzer.
[5+5+6]
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8. Describe the process of manufacture of citric acid with a flow sheet indicating the material and energy flow at every point. [16]
1
Code No: 07A42304
R07
Set No. 4
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II B.Tech II Semester Examinations,December 2010 BIOPROCESS ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Write a note on medium design and usage.
[16]
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2. Write the step -wise procedure to calculate the heat of reaction with oxygen not as a principal electron acceptor. [16] 3. What is solid-state fermentation process? Explain, how enzymes produced using this process? [16]
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4. Estimate the theoretical growth and product yield coefficients for ethanol fermentation by S. cerevisiae as described by the following reaction C6 H12 O6 → 2C2 H5 OH + 2CO2 . [16] 5. Describe various requirements of a fermentation process with a flow sheet.
[16]
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6. Enumerate difference between growth and non-growth associated product kinetics. [16] 7. Write a brief note on biosensors, and describe how they are used for measuring process parameters. [16] 8. For many fermentation processes, fed - batch conditions are preferred over continuous feeding conditions - Discuss this with some specific examples. [16]
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2
Code No: 07A42304
R07
Set No. 1
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II B.Tech II Semester Examinations,December 2010 BIOPROCESS ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. How the oxygen requirements of an industrial fermentation medium are met? Describe with examples. [16]
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2. Explain the growth energetics with relation to microbial growth giving relevant equation supporting it. [16]
3. Describe various physical process parameters that need to be controlled in a fermentation process. Explain them briefly. [16]
or
4. Describe the processes of precipitation of cellular extract and discussing its advantages and disadvantages. [16]
5. Explain the procedure involved in the determination of cell number density and cell mass concentration. [16]
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6. Enumerate the range of fermentation process. Explain with examples.
[16]
7. Derive the expression for determining the maintenance coefficient in continuous cultivation. [16] 8. What is meant by minimum fluidization velocity? What is its importance in design of fluidized bed bioreactors? [16]
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3
Code No: 07A42304
R07
Set No. 3
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II B.Tech II Semester Examinations,December 2010 BIOPROCESS ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Explain the following:
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(a) Elemental balances (b) Available electron balances (c) Maintenance coefficient.
[6+5+5]
2. Write a note on aerobic fermentation processes with specific examples.
[16]
4. Write short notes on: (a) Filtration (b) Centrifugation (c) Coagulation and Flocculation
or
3. Explain in detail about the general unsteady state energy balance equations. [16]
[5+5+6]
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5. (a) How the minerals requirements of an industrial fermentation medium are met? Describe with examples. (b) What are various nutrients required in the formulation of an industrial medium, and how they are met? [8+8]
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6. (a) Explain the Monod model and state the assumptions and limitations of it. (b) In a chemostat, if a culture obeys the Monod equation the residual substrate is independent of the feed substrate concentration. But in an experiment it was observed that the residual substrate concentration increases as the feed substrate concentration is increased. Instead of monod equation apply contois equation and derive an expression for S in terms of So, D, Ksx, YMX/S and mm. State whether the residual substrate concentration increases with feed substrate concentration as observed in the experiment and calculate how much increase in residual substrate concentration is expected if the feed substrate concentration is increased two fold. [8+8]
7. Describe how a computer can be coupled to a fermenter for controlling various process parameters. [16] 8. What are various steps involved in converting the idea of a product into a commercial reality? [16] ????? 4
Enumerate difference between growth and non-growth associated product kinetics. [16] · 7. Write a brief note on biosensors, and describe how they are used for ...
(a) What is Radix-64 format? Explain how both PGP and S/MIME perform the · Radix-64 conversion is performed. (b) Describe the five principal services that ...
Farm in Maharashtra, supplied with water from a dug well pumping for 4 h a day, are given below. Determine the required discharge. [16]. 7. AjntuWorld.in ...
... carry equal marks · ***** · 1. List eight major functions of an exhaust nozzle. .... plane. Show the internal layout for swallowed air to reach the engine. Explain.
(b) A turbine is to operate under a head of 30 m at 300 r.p.m. The discharge is ... 2. (b) An inward flow reaction turbine has an external diameter of 1 m and its.
(a) Discuss the response of high pass RC circuit for square wave input. (b) A 10Hz square wave is fed to an amplifier. Calculate and sketch the output wave ...
[16] · 8. With a neat sketch discuss the working procedure of neutron radiography, and also · give applications, advantages and limitations of this method. [16] · *****. 1 ... 5. (a) Give the limitations of x-ray diffraction method and ultrasonic met
tetrachloride during indirect chlorinate of TiO2 at 1020 0C · [16] · TiO2(g) + 2Cl2(g) = TiCl4(g) + O2. 8. (a) How do the interstitial elements affect mechanical properties of commercially · pure titanium? (b) Why is 0.2 % Pd added to titanium? What
register machine.(Hint: Use FNS). [8+8] · 5. (a) Explain the strassen's matrix multiplication · (b) Derive the time complexity for binary search. [8+8] · 6. (a) Find an ...
Variance · (b) The mean and variance of a binomial distribution are 2 and 8 / 5. Find ... marks were normally distributed with mean 70 and standard deviation 15.
Computer Science And Engineering · Time: 3 hours · Max Marks: 80 · Answer any FIVE Questions · All Questions carry equal marks · ***** · 1. (a) Distinguish between a packet-filtering router and a stateful inspection firewall? (b) âOne way to secure
1. With the help of a neat diagram indicating the various geometric elements of a · traffic rotary ... sight distance. What is the need to have SSD and factors.
2. Explain the alpha, delta, theta and beta waves emerging from the brain. [16] · 3. (a) Explain in detail on the isolation amplifiers used in medical field and its need ... Discuss the generation and propagation of action potential in a cell. [16] ·
(a) Steam pressure in a reactor heating coil. (b) Flow rate of reactants into a polymerization tank. (c) Flow of effluent from a waste water treatment holding tank into a river. (d) Flow of cooling water to a distillation condenser. [16] · 6. (a) Def
(a) State Newton's law of viscosity and give any four examples of its applications. (b) An oil of viscosity 8 poise is used for lubrication between a shaft and sleeve.
(a) Off - peak load is zero ... load current and p.f 0.1. capacitors are supplied to make the line power factor ... (b) The annual peak load of substation is 3500kW.
Set No. 2. (a) Construct a systematic (15,2) code using the generator polynomial .... taken. The bit rate is 'rb' and the channel noise has a PSD Gn(f) given by.
Discuss the principle of measuring the hardness of Heat Affected Zone using Jominey · hardenability test? .... (b) What is post weld heat treatment? Describe the ...
(a) State Darcy's law. Discuss the assumptions and limitations. .... 1. (a) State Stoke's law and explain its limitations as applicable to the sedimentation · analysis.
[4+4+4+4] · 4. Add a new method in the base class of Shapes.java that prints a message, but don't .... (d) Dynamic binding and message passing. [4+4+4+4].
Discuss clustering and solubility of liquid mixture. [16] · 3. Define: ... no adsorption at the pore wall and for the case with monolayer adsorption at the · pore wall.
Calculate the depth of indentation produced on a glass surface in ultrasonic ma- · chining by the throwing action of abrasive grain of 100 µm diameter.
