H6U1/8A16

Reg. No St. Joseph’s College of Arts & Science Autonomous) St. Joseph’s College Road, Cuddalore – 607001 BC612-BIO TECHNOLOGY Time: 3 hrs

Max Marks: 75 SECTION-A (25X1=25) Answer ALL the questions: Choose the best answer (10X1=10) 1.

The most popular and widely used engineered vector is (a) PBR322 (b) PUC vector (c) Psc 101 (d) Puc 19

2.

Restriction enzymes are also called (a) molecular knives (b) molecular scissers (c) molecular scalpels (d) all of these

3.

Protoplast are the cells devoid of (a) cell membrane (b) cell wall (c) both cell wall and cell membrane (d) none of these

4.

Bt Cotton is a) Cloned plant b) Transgenic plant c) Hybrid plant d) Mutated plant

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5.

What are the main constituents of culture for animal cell growth? a) Glucose and Glutamine b) Growth factors c) Cytokines d) All of these

6.

Cells which have undergone transformation frequently become a) anchorage independent b) anchorage dependent c) stable d) unstable

7.

pH of culture medium is initially controlled by a) presence of CO2 b) presence of bicarbonate buffer c) addition of bases d) none of these

8.

9.

The main reason for production of antibiotics in fed batch reactors is a)

the presence of precursors is often toxic to the cells

b)

higher yields when cells enter the stationary phase

c)

higher yields when cell growth slows

d)

all of the above

Androgenic Plants developed in vitro culture from ________ (a) pollen grain (b) anther (c) ovule (d) flower

10. _________________ is following open system. (a) batch culture

(b) fed batch culture

(c) Continuous culture (d) none

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Say true or false

(5X1=5)

11. PBR 322 is the most common plasmid vector used in genetic engineering 12. Subunit recombinant vaccines are the components of the pathogenic organisms. 13. DMEM is one of the complete medium 14. It is now possible to breed plants and animals with desired characters through Chromosome engineering 15. Chemical compounds that do not participate in metabolism of plants are called primary metabolites. Answer in one sentence

(10X1=10)

16.

Define YAC

17.

What is a chimeric mouse?

18.

Define cell line

19.

What is suspension culture

20.

What is a cloning vector?

21.

What are recombinant vaccines?

22.

Give examples for media.

23.

What are Haploids?

24.

What are spargers?

25.

Give difference between fermenter and bioreactor SECTION – B (4X5=20) Answer any FOUR of the following:

26. What are plasmids? How do they work as cloning vector? 27. What are the advantages and disadvantages of adding serum in the animal culture medium?

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28. What are cell lines? How is the growth characteristics of cell lines determined? 29. List out the applications of transgenic animals. 30. What is micropropagation? Explain briefly. 31. What is a fermenter? How does it function? SECTION –C (3X10=30) Answer Any THREE of the following: 32. What is bioprocess technology? Classify. 33. How Hepatitis B Vaccines are produced? 34. Explain the role of Ti plasmid in gene transfer. 35. Write in detail about production and applications of monoclonal antibodies. 36. Elaborate protoplast culture.

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