Animal Free Recombinant Human FGF-10 Catalog Number: 100-183AF

Shenandoah's Animal Free (AF) Proteins are created with ANIMAL FREE RAW MATERIALS and ANIMAL FREE PROCESSES.

Product Description_____________________________________________________________________ Alternate Names: Keratinocyte growth factor 2 Accession Number: O15520 Description: Fibroblast Growth Factor 10 (FGF-10) is a growth factor involved in a broad range of functions that include morphogenesis, proliferation, embryonic development, tissue repair and more. Source: E.coli MW: Non-glycosylated protein containing 170 amino acids, with a total molecular weight of 19.2 kDa. AA Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Product Specifications*__________________________________________________________________ *Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are typical values to be expected for this product.

Formulation: Lyophilized from a sterile filtered aqueous solution containing 10 mM Na3PO4, pH 7.5 Purity: Purity is typically 95% as determined by reducing and non-reducing SDS-PAGE Endotoxin Level (kinetic LAL analysis): Typically less than 0.05 EUs/μg protein (50% confidence range) Biological Activity: The activity is determined by a proliferation assay using 4MBr‑5 rhesus monkey epithelial cell and is typically 20-100 ng/mL. This corresponds to an expected specific activity of 5 x 104 units/mg.

Preparation and Storage_________________________________________________________________ Country of Origin: USA Shipping: Room temperature Storage Prior to Reconstitution: -20ºC Product Reconstitution: Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws. Expiration Date:  12 months from date of receipt when stored at -20 to -80 ºC as supplied.  1 month when stored at 4 ºC after reconstituting as directed.  3 months when stored at -20 to -80 ºC after reconstituting as directed.

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!