Set No. 1

Code No: RR312304

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III B.Tech I Semester Supplimentary Examinations, November 2008 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What is a medium? Explain the preparation of common tissue culture medium. [6+10]

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2. What are alkaloids? Explain the process for the production of alkaloids? [4+12] 3. Write about shikonin production at commercial level?

[16]

5. Write a short notes on any two (a) CaMV genome (b) TMV genome

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(c) Plasmids as vectors

or

4. What are the different types of transformation techniques that are used for the production of transgenic plants? [16]

[8+8]

6. Explain the current status of Nitrogen fixation in the field of Biotechnology? [16] 7. Write a note on phenotypic and genotypic effects of transposition?

[8+8]

8. Explain the genetic engineering mechanism behind the development of stress tolerant plants. [16]

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Set No. 2

Code No: RR312304

1. Discuss the importance of Plant tissue culture in India?

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III B.Tech I Semester Supplimentary Examinations, November 2008 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ [16]

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2. What is a plating technique? Explain its applications in the production of secondary metabolites? [6+10] 3. Explain the factors effecting growth in a bioreactor?

[16]

4. What is the importance of Vir genes in the Agrobacterium mediated gene transfer? [16] [16]

6. What is meant by Symbiosis? Explain it with a suitable example.

[6+10]

or

5. Write about general characteristic features of plant viral vectors?

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7. Barbara Mc Clintock discovery of colour change in maize OF great significance in modern genetics - Justify. [16] 8. Write short notes on any two (a) Virus resistant plants (b) Bt endotoxin

(c) Transformation

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[8+8]

Set No. 3

Code No: RR312304

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III B.Tech I Semester Supplimentary Examinations, November 2008 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write a note on totipotency? Explain the importance of organogenesis?

[6+10]

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2. Describe the secondary metabolites of natural origin with special reference to alkaloids. [16] 3. What are methods of augmenting cell biomass in bioreactors?

[16]

4. How Ti plasmid transfers T-DNA in host cells?

[16]

6. Write short notes on any two (a) Nitrogenase

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(b) Ferridoxin

or

5. How the plant viruses are classified and what is the importance of viruses in the foreign gene integration? [6+10] [8+8]

(c) Leghemoglobin

7. What is meant by disease resistance and Susceptibility? 8. Give an account on the herbicide resistant plants.

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[8+8] [16]

Set No. 4

Code No: RR312304

1. Discuss the importance of Plant tissue culture in India?

in

III B.Tech I Semester Supplimentary Examinations, November 2008 PLANT BIO-TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆

3. Write about the physical processes that need attention in a bioreactor? 4. Write short notes on any two

(a) CaMV genome

uW

(b) TMV genome

or

(b) T- DNA transfer

5. Write a short notes on any two

[16]

[8+8]

(a) Vir gene (c) Selectable marker

[16]

ld .

2. Describe the various strategies used for secondary metabolites synthesis.

[16]

[8+8]

(c) Plasmids as vectors

6. What is symbiosis? Explain the process of symbiosis in legumes. 7. How do transposons induce deletions? Inversions? 8. Write short notes on any two

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(a) Protinase inhibitors

(b) Recombinant baculovirus

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(c) Insect control agents

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[6+10] [8+8] [8+8]