II B.Tech II Semester Regular Examinations, Apr/May 2009 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆
2. Write on: (a) Meselson and Stahl Experiment (b) Taylor, Woods and Hugh’s Experiment.
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1. What was the procedure used by Altman in 1889 to isolate protein free nucleic acid from yeast and animal tissues? Explain his method. [16]
[8+8]
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3. RNA is a template for DNA telomerase. Discuss the statement with evidences. [16]
4. Discuss the synthesis of r RNA in eukaryotes. Explain using TF attachment sites. [16]
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5. “Some heat shock protein genes that act rapidly to protect other proteins from harsh conditions”. Explain this statement describing the mechanism that has evolved to regulate the expression of such genes. [16] 6. What is an anticodon and what is its function?
[16]
7. Describe the processes and role of different components in the chain elongation and chain termination steps of protein synthesis. [16]
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8. Contrast and compare the mutagenic effects of deaminating agents, alkylating agents and base analogous. [16]
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Set No. 2
Code No: 07A42303
in
II B.Tech II Semester Regular Examinations, Apr/May 2009 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆
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1. DNA is made with both single (SS) and double strands (DS) of bases. Which procedure will help to confirm that the DNA is having either ss or ds or both? Write the reasons. [16] 2. Write a brief account on rolling circle, explication of DNA. 3. Explain the important events in cell cycle.
[16]
[16]
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4. How does mutations helps in the elucidation of transcription apparatus? Discuss. [16]
5. What is the evidence that an mRNA exporter directs mRNPs through the nuclear pores? Give evidence with reference to the vertebrate system. [16] 6. Describe the following terms:
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(a) ribosome binding site (b) RNA polymerase,
(c) tRNA synthetases.
[5+5+6]
7. Describe the differences observed in the initiation of translation between prokaryotes and eukaryotes. [16]
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8. What are frame shift mutations? How does it influence the gene product, explain giving examples? [16]
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Set No. 3
Code No: 07A42303
in
II B.Tech II Semester Regular Examinations, Apr/May 2009 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) What are the typical values of DNA content in different organs of human body?
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(b) How a plant and animal tissue will be prepared for the isolation of RNA? (c) Explain the principle of submerged electrophoresis of DNA. (d) Write on the principle of autoradiography.
[4×4] [16]
3. Describe the steps in formation of prereplicative complex.
[16]
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2. List out the various enzymes involved in DNA repliation of Prokaryotes.
4. Eukaryotes contain three types of RNA polymerases? What are they? List their variations. How were they discovered? [16]
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5. How are the rRNAs derived from the primary transcripts in E.coli? 6. Can more than one ribosome translate the same mRNA transcript?
[16] [16]
7. Explain the statement “The steps of protein synthesis are basically the same in eukaryotic cells as in prokaryotes. The ingredients, however, can be different”. [16]
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8. Describe tautomerism and the way in which this chemical event may lead to mutation. [16]
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Set No. 4
Code No: 07A42303
in
II B.Tech II Semester Regular Examinations, Apr/May 2009 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆
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1. (a) In 1954 CF Crompton and his colleagues used to isolate a procedure for the preparation of nucleoprotein from thymus gland. Discuss the principle of this procedure. (b) What are the differences between nucleoproteins and nucleohistones?
[8+8]
2. What are the methods used to establish that the DNA synthesis is unidirectional or bi-directional in prokaryote? Discuss your answer with details of experiments. [16]
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3. Explain briefly about replication errors in prokaryotes and its repair.
[16]
4. How does mutations helps in the elucidation of transcription apparatus? Discuss. [16]
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5. What processing events occur with prokaryotic mRNA?
[16]
6. Describe the structure of t-RNA and give its functional role in protein biosynthesis. [16] 7. What are the different inhibitors of protein synthesis?
[16]
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8. Explain how the system recognizes progeny strand rather than parent strand as one with mismatch? [16]
Discuss the synthesis of r RNA in eukaryotes. Explain using TF ... DNA is made with both single (SS) and double strands (DS) of bases. Which · procedure will ...
(a) How do you explain tremors in hands ? (b) List out the types of tremors in hands. (c) What mechanical apparatus will suit the worker with tremors ? 7. Design ...
... the velocity of block A after it has moved 2 m. Assume that µ equals to 0.25 between block A and the plane and that the pulley · is weightless and frictionless.
(a) Differentiate fully between welding, brazing and soldering. (b) What is a flux? What is the use of a flux in a brazing process? Explain the various fluxes used ...
(b) An optical fibre has a NA of 0.20 and cladding refractive index of 1.59. De- · termine the refractive index of core and the acceptance angle for the fibre in.
Write how blood PH is maintained. [16]. *****. 1 of 1 ... [6+5+5] · 8. Explain how blood biochemical analysis helps in confirmation of diseases. [16] · *****. 1 of 1.
(c) Toughness and resilience · (d) Endurance limit and fatigue strength · [4x4=16] · 6. Sketch time-strain curve for anelastic behavior and compare it with the time- ...
(b) Radiation carcinogenesis · 6. Discuss the various radiaton protection measures ? [16] · 7. (a) Explain how Nuclear Medicine is useful in the imaging of CNS?
Why does the Law of Diminishing Returns operate? Explain with the help of · assumed data and also represent in a diagram. [4+6+6] · 3. A company reported ...
1. Describe how the nutritive values of food can be sustained by using Biotechnology ... 4. Explain in detail how ultraradiation is used in food preservation? [16].
for the magnetic fields in the different regions of a coaxial cable, having inner .... Draw the directions of electric and magnetic fields in parallel plate and coaxial.
8. Discuss the different waves and rhythms in Electroencephalogram. ... 8. What are the alpha and beta rhythms in EEG? Give their normal frequency range.
[10] · (b) Write a note on âaskarelsâ. [6]. 8. Give an account of the following properties of lime. [4x4=16]. (a) Slaking. (b) Setting and hardening of lime. 1 of 2.
Give various examples of capital budgeting decisions. Classify ... or other unit of Management among the activities of that unit. Explain. .... 100000 Fixed Assets.
(b) Three Brick wall in English bond. [8+8] · 6. (a) Name the different types of foundations you would recommend under different · situations and soils. Explain ...
lakhs and average variable cost of Rs.10 each . The selling price is to yield 20% · profit on cost. you are required to calculate · (a) P/V ratio. (b) Break even point.
i. crystal structure factor ... (a) Define and explain space lattice, unit cell and crystal. ... (b) What factors control the Quenching characteristics of liquid coolants?
(a) What is meant by page pinning in the buffer pool. Who is responsible for .... (b) Discuss the techniques for allocating file blocks on disk. [8+8] · 4. Consider the ...
Code No: RR310402. Set No. 1 ... it in the long distance transmission of radio signals in the HF band. 1 of 2 ... iii. Beam area, beam efficiency and resolution.
PLANT BIO-TECHNOLOGY ... 1. Discuss the importance of Plant tissue culture in India? [16] ... 7. What is meant by disease resistance and Susceptibility? [8+8].
(a) What are the factors that lead to fading and attenuation in ionospheric prop- agation. (b) What is the wave tilt and how does it effect the field strength received ...
3. (a) How do you detect whether an organic reaction is going by free-radical mech- · anism or not ? (b) Describe the chlorination of ethane in the presence of ...
