Set No: Code No: RR- 312302 III B.Tech I-Semester Regular Examinations, November-2004 GENETIC ENGINEERING (Bio- Technology) Time: 3 hours Max. Marks: 80 Answer any FIVE questions All questions carry equal marks --Discuss the regulatory components of the lac operon system.
2.
Describe the mechanism of gene regulation in eukaryotes with a suitable example.
3.
What are the characters of ideal plasmid. Explain with suitable example.
4.
Give the methods of isolation and purification of DNA from plant systems.
5.
Give a brief definition of a gene library. What is the essential difference between a genomic library and a cDNA library? List the major advantages/limitations on the use of each.
6.
What is meant by PCR? Describe its methodology.
7.
What is RFLP? Citing suitable examples, highlight its use in gene mapping.
8.
Discuss about the viral methods used in doing In vivo gene therapy.
or
ld .
in
1.
1
Aj
nt
uW
^*^*^
Set No: Code No: RR- 312302 III B.Tech I-Semester Regular Examinations, November-2004 GENETIC ENGINEERING (Bio- Technology) Time: 3 hours Max. Marks: 80 Answer any FIVE questions All questions carry equal marks --Explain the role of promoter and operator in lac operon.
2.
Explain the structural and functional details of DNA binding regulatory proteins in eukaryotes.
3.
What are artificial plasmids? How can they be produced?
4.
Explain the role of enzymes involved in genetic engineering.
5.
Write the steps involved in the construction of a cDNA library.
6.
How is PCR different from the traditional cloning?
7.
Write short notes on any two: a) Satellite markers b) Restriction enzymes c) Gel electrophoresis
8.
Write about the various steps involved in gene therapy.
ld .
or
uW nt
^*^*^
Aj
in
1.
2
Set No: Code No: RR- 312302 III B.Tech I-Semester Regular Examinations, November-2004 GENETIC ENGINEERING (Bio- Technology) Time: 3 hours Max. Marks: 80 Answer any FIVE questions All questions carry equal marks --Write notes on any two of the following: a) Repressor b) Inducer c) Effector
2.
What is signal transduction? How does it help in regulating gene expression?
3.
Write notes on any two: a) Relaxed plasmid b) PUC8 c) - Complementation
4.
Describe the types of restriction enzymes and their functions.
5.
Discuss in detail the nucleic acid blotting techniques used in detection of cloned genes.
6.
How are PCR products identified?
7.
Using suitable examples, explain the importance of 16s r RNA as a molecular marker.
8.
Write about the non-viral methods used for DNA injection in gene therapy.
uW
or
ld .
in
1.
nt
^*^*^
Aj
3
Set No: Code No: RR- 312302 III B.Tech I-Semester Regular Examinations, November-2004 GENETIC ENGINEERING (Bio- Technology) Time: 3 hours Max. Marks: 80 Answer any FIVE questions All questions carry equal marks --Write notes on any two of the following a) Promoter b) Operator c) Structural genes.
2.
Describe the regulation of gene expression in immunoglobulin production.
3.
Explain the mechanism of transposition.
4.
Describe the methods of manipulating DNA for gene cloning purpose.
5.
Write short notes on any two: a) Western blotting b) Reverse transcription c) Probes
6.
Write short notes on any two: a) Primers in PCR b) Taq polymeraoe in PCR c) cDNA
7.
How is RAPD used in molecular diagnostics?
8.
What is “Molecular pharming”? What are the gene products, which can be obtained from transgenic animals?
Code No: RR- 312302. III B.Tech I-Semester Regular ... Write about the non-viral methods used for DNA injection in gene therapy. ^*^*^. Set No: 3. AjntuWorld.in ...
List out the practical considerations to be checked for continuous gas ... Explain how silica analysis is done in boiler water of power plant. 3. a) What is meant by ...
What do you understand by principles of planning a building? Prepare a typical · aspect diagram for the area where direction of prevailing breeze is from North-.
b) Draw the circuit diagram of 4-bit Johnson counter using D-flip flop and explain .... 6. a) Compare merits and demerits of ripple and synchronous counters.
Given: turbine diameter = 120m and turbine operating speed = 40 rpm at · maximum efficiency. Propeller type wind turbine is considered. 4. a) What is the basic ...
Obtain an expression for unit speed, unit discharge and unit power for a turbine. 2. a) What do you understand by air vessels? Explain with a neat sketch.
1.a) Obtain the expression for the resistance and capacitance for gas system. ... List various variables to be measured and controlled in nuclear power plant.
instructions at the beginning and end of this set of code in order to switch .... 5.a) Write an assembly language program that will examine an ASCII string of 100.
If the analog i/p voltage is +6.85 v, what will be the R-2R ladder · o/p voltage when ... b) Design a ramp generator using 555 timer having an output frequency of.
efficiency of the cycle and mean effective pressure for the cycle. ... b) Calculate the heat flux emitted due to thermal radiation from a black surface at · 6000.
8.a) Write a note on creep resistant materials. b) Explain all the ... b) Sketch the creep diagram, and explain the various stages of creep. @@@@@. Set No. 2.
What is the difference between radio compass and ADF? Explain the principle · and operation of Automatic direction finder. 6. Define analog and digital ...
endurance limit of a metal. b) What are the factors which affect the fatigue strength of materials? 7. a) What is creep? Draw an ideal creep curve and explain the ...
(i) Band Rate · (ii) Request Filter ... 3. a) Write the comparison between teleprinters and CRT terminals. b) What are the ... What savings does it offer? b) What is a ...
b) How does butadiene differ from isoprene? 4.a) Describe about the synthetic fiber industry in India. b) Write, with an example on each of the following.
graph. b) Draw the state space tree for m-closing graph using a suitable graph. 8. Present a program schema for a FIFO Branch & Bound search for a Least-Cost.
b) Explain the effect of Metallurgical variables on fatigue properties · 7.a) Explain Ductile to Brittle transition phenomenon. b) Compare charpy with Izod impact testing. 8.a) What is stress relaxation. Draw stress-strain curves for ductile and brit
Information Technology, Electronics and Control Engineering, Electronics and ... Computer Engineering, Computer Science and System Engineering) ..... Draw and explain the logic diagram of a master slave D flip-flop using Nand gates. ###.
Chemical Engineering, Mechatronics, Metallurgy & Material Technology, · Aeronautical ... b) What is cache memory? Where is it placed? What is its .... 7.a) Explain the steps involved in computing the coefficients of the regression · polynomial.
b) Classify the blast furnace stoves. What are the different values in the stove and · their functions? 3.a) What factors affect reducibility of iron ores? Discuss them.
1.a) Write the name of places for occurance of iron ores in India. b) Explain the process of either Sintering or Pelletizing. 2.a) Explain hearth, tuyeres, stack with ...
