III B.Tech I Semester Regular Examinations,Nov/Dec 2009 GENETIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. (a) What is lac-operon?
[10+6]
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(b) Define sigma factors 2. Write a account on DNA hybridization techniques.
[16]
3. Comment on the applications of molecular markers in various fields of Biotechnology. [16]
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4. Describe the methods of manipulating DNA for gene cloning purpose.
[16]
5. What are transposons? and describe about the types of bacterial transposons. [16] 6. Discuss about multiplex PCR.
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7. Enumerate the different methods of gene transfer?
8. Give a detailed account on repetitive DNA and their applications.
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1
[16] [16] [16]
Code No: 45055
R07
Set No - 2
1. Explain the principle, methods and applications of PCR.
[16]
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2. Write short notes on:
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III B.Tech I Semester Regular Examinations,Nov/Dec 2009 GENETIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
(a) Promotors (b) Enhancers
[8+8]
[16]
4. How is tissue specific gene regulation achieved in eukaryotes?
[16]
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3. How the plassmids can be identified? and how they can be purified?
5. Discuss in detail about the construction of molecular maps of animal genome. [16] 6. Explain in detail about Tryptophan operon
[16]
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7. List the different enzymes involved in genetic engineering and its applications? [16] 8. Write the steps involved in construction of cDNA library.
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2
[16]
Code No: 45055
R07
Set No - 3
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III B.Tech I Semester Regular Examinations,Nov/Dec 2009 GENETIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Write short notes on:
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(a) λ-phage (b) Cosmids.
[8+8]
2. Explain in detail about gene amplification and its uses?
[16]
3. Write note on 16S-rRNA typing and its applications.
[16]
or
4. Discuss in detail the nucleic acid blotting techniques used in detection of cloned genes. [16] 5. Explain in detail about Arabinose operon.
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6. Write short notes on
[16]
(a) primers in PCR
(b) Taq polymerase in PCR (c) c DNA
7. Give a brief account on mechanism of transposons.
[5+5+6] [16]
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8. Write about the non-viral methods used for DNA injection in gene therapy. [16]
3
Code No: 45055
R07
Set No - 4
1. Give brief account on applications of gene cloning.
[16]
[16]
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2. Compare & contrast between PCR and RT-PCR.
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III B.Tech I Semester Regular Examinations,Nov/Dec 2009 GENETIC ENGINEERING Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
3. Write short notes on: (a) Repressors (b) Activators
[8+8]
or
4. In r-DNA technology, what are the different steps involved in cloning-Explain with the help of example. [16] 5. What is DNA sequencing? Give detailed account of MAxam-Gilbert method of DNA sequencing. [16]
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6. (a) what are Retrotransposons?
(b) Give the applications of transposons?
[8+8]
7. Describe in detail on Gene regulation in the eukaryotic system and its functions? [16] 8. Write short notes on
Describe the methods of manipulating DNA for gene cloning purpose. [16] · 5. What are transposons? and describe about the types of bacterial transposons. [16].
2. (a) Explain why hardening by quenching is followed by tempering. (b) Only mechanical properties not physical properties can be altered by heat · treatment.
Young's modulus = 35 Ã 1010 Pa and the specific surface energy = 2 J/m2 · (b) Give a brief account on the various stages of creep rate with reference to design.
All Questions carry equal marks · ***** · 1. Explain the microbial production of aminoacids with one example. [16] · 2. What are biofuels? How these are produced ...
1. Explain tree based indexing and hash based indexing techniques. [16] · 2. Explain the .... 1. Explain in detail the structure of B+ tree with an example · [16].
what do you understand by Lubrication of Joints? [16] · 5. (a) Explain the three features of viscoelasticity in detail · (b) Explain the working principle of oscillating ...
Perform a Chi-square test at .o5 level whether there is a significance difference ... (b) Derive formulae to find the mean and variance of the Binomial distribution.
and T1 = sink temperature) for a Carnot refrigerator whose COP is 5. Also ... fusible plug, at what temperature should the plug melt to limit the maximum.
[8+8] · 7. (a) List out equipments used in pathology and blood bank departments of a · hospital. (b) Discuss about various blood groups present in humanbeings.
medical equipment. (b) Write a note on responsibilities of BME Department. [8+8] · 8. Discuss the sanitation procedures and laundry services with in a hospital.
Define and derive the equation for the vorticity of a flow. Hence prove that ... out from the leading edge to the trailing edge of a symmetrical airfoil. (b) State and ...
Check whether the following impedance functions are LC, RL or RC? (a) Z(s) = ... amplitude 5 V. Find autocorrelation function and energy spectral density. [8+8].
3. Regeneration is possible in the fixed-bias transistor flip-flop if the base-to-base · voltage gain exceeds unity. Verify that this gain condition is satisfied provided ...
Set No - 2. (b) Write about bus structures. ... (b) Explain time-shared common bus in detail. [8+8] ... (c) Asynchronous serial transmission with one stop bits · [16].
Consider a telegraph source having two symbols, dot and dash. The dot duration · is 0.2s.The dash duration is 3 times the dot duration. the probability of the dots.
(a) Explain how one can test for homogeneous catalyzed reaction using integral · method of Analysis. (b) An ampoule of radioactive Kr- 89 (half life = 76 minutes ) ...
(c) Cell Suspension cultures. ... 6. Explain the process of producing secondary metabolites in culture. ... (a) Discuss the importance of plant tissue culture in India.
3. Effect of radiation on Bone Marrow. (a) Describe the ... (b) Write short notes on artificial respiration. [8+8] ... (b) Write a note on Meta static bone diseases. [8+8].
(a) Explain the terms : Compilers, Linkers, Loaders, Assemblers. (b) Describe the program execution in a system with a neat diagram. [8+8] · 4. (a) Explain Read ...
(c) Explain why XML uses both streaming and tree-based parsing. [4+7+5] · 2. Explain the functionality of javax.servlet package for web servers. Discuss about.
of the offset point D on the coupler AB, and the angular velocities of links OA and ... lengths AB : OO1 : OA :: 1 : 2 : 2.5 for the point P to move in an approximate.
