III B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOCHEMICAL REACTION ENGINEERING-1 Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Write short notes on
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(a) enzyme specificity (b) active site
[8+8]
[16]
3. Describe about the product formation stoichiometry of fermentation.
[16]
4. Derive the design equation for a batch reactor. Write the advantages and disadvantages using batch reactors in bioprocessing?
[16]
or
2. Explain the mass transfer limitations in immobilized enzyme systems.
5. Explain the significance of type of carrier, concentrations, pH, temperature, and reaction time in immobilization of enzymes. [16]
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6. Explain the concept of search for a biochemical reaction mechanism taking enzyme-substrate as example.
[16]
7. Given that the maximum specific growth rate of an organism is µmax = 1 h-1 and its Ks = 1.5 g/cm3. If the input substrate concentration is 145 g/cm3 and concentration of product at this maximum output rate is 10 g/cm3, determine the maximum output rate of the product. [16]
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8. Discuss in detail about applications of RTD Measurements.
1
[16]
Code No: 45053
R07
Set No - 2
1. What is Han-Levenspiel model? Explain it in detail.
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III B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOCHEMICAL REACTION ENGINEERING-1 Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? [16]
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2. Describe about online data analysis for measurement of biochemical parameters. [16] 3. Explain the concept of enzyme stability and immobilization?
[16]
4. Derive an expression for the concentration profile of a substrate following first order kinetics and spherical geometry considering internal mass transfer. [16]
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5. Michaelis-Menton kinetics are used to describe intracellular reactions. Yet [E0 ] ≈ [S0 ]. In invitro batch reactors, the quasi steady state hypothesis does not hold for [E0 ] ≈ [S0 ]. The rapid equilibrium assumption will not hold. Explain why Michalis-Menten kinetics and the quasi-steady-state approximation are still reasonable descriptions of intracellular enzyme reactions. [16] 6. (a) What are the factors that contribute to increase specific growth rate? (b) A batch fermenter is being used for the production of yeart cell from glucose. The number of yeart cells is found to double in 1.5h. Find the specific growth rate of cells. [8+8] 7. (a) Explain about various variables affecting the rate of biochemical reaction. (b) Describe various methods of expressing rate of a biochemical reaction. [8+8]
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8. “RTD as a diagnostic tool for detecting and characterizing flow behavior” Explain. [16]
2
Code No: 45053
R07
Set No - 3
1. Describe about the cell growth stoichiometry of fermentation.
in
III B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOCHEMICAL REACTION ENGINEERING-1 Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? [16]
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2. Describe Typical batch growth curve of a microbial culture. Explain about various growth phases in the curve. [16] 3. Discuss the various physical and chemical techniques used for enzyme immobilization.
[16]
4. (a) Describe the effect of substrate on growth inhibition.
or
(b) Describe the effect of product on growth inhibition.
[8+8]
5. (a) Explain how activation energy and temperature dependency are interdependable for a biochemical reaction. (b) Explain the role of Arrhenius law on a bio chemical reaction.
[8+8]
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6. Establish relation ship between the equilibrium constant and its relation to G0 .[16] 7. Write short notes on
(a) ENZYME CATALYSIS
(b) Nature and Examples of Enzyme Catalysis
[8+8]
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8. You are working for a company A and you join a research group working on immobilized enzymes. Harry, the head of the lab, claims that immobilization improves the stability of the enzyme. His proof is that the enzyme has a half life of 10 days in free solution, but under identical conditions of temperature, pH , and medium composition, the measured half life of a packed column is 30 days. The enzymes immobilized in a porous sphere 5 mm in diameter. Is Harrys reasoning right? Do u agree with him? Why or why not? [16] ?????
3
Code No: 45053
R07
Set No - 4
1. Explain about overall growth stoichiometry of fermentation.
in
III B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOCHEMICAL REACTION ENGINEERING-1 Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? [16]
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2. Derive the design equation for a CST reactor. Write the advantages and disadvantages using batch reactors in bioprocessing? [16] 3. Explain the application of immobilized enzymes in industry and medicine.
[16]
or
4. Milk is pasteurized if it is heated to 630 C for 30 min, but if it is heated to 740 C it only needs 15 s for the same result. Find the activation energy of this sterilization process? [16] 5. Disuss in detail about the effects of temperature and pressure changes on the equilibrium constant for a reaction. [16]
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6. Given that the maximum specific growth rate of an organism is max = 1 h-1 and its Ks = 1.5 g/cm3. If the input substrate concentration is 145 g/cm3, determine the maximum output rate for this system in a CFSTR. [16] 7. Derive Michaelis-Menten reaction rate expression with different assumptions. (a) Equilibrium Assumption
(b) Sequential irrevesible Reaction Assumption (c) Quasi-Steady State Assumption
[5+5+6]
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8. Discuss the external diffusional effects on the immobilized enzyme kinetics on non-porus supports. [16]
what do you understand by Lubrication of Joints? [16] · 5. (a) Explain the three features of viscoelasticity in detail · (b) Explain the working principle of oscillating ...
Perform a Chi-square test at .o5 level whether there is a significance difference ... (b) Derive formulae to find the mean and variance of the Binomial distribution.
and T1 = sink temperature) for a Carnot refrigerator whose COP is 5. Also ... fusible plug, at what temperature should the plug melt to limit the maximum.
[8+8] · 7. (a) List out equipments used in pathology and blood bank departments of a · hospital. (b) Discuss about various blood groups present in humanbeings.
medical equipment. (b) Write a note on responsibilities of BME Department. [8+8] · 8. Discuss the sanitation procedures and laundry services with in a hospital.
Define and derive the equation for the vorticity of a flow. Hence prove that ... out from the leading edge to the trailing edge of a symmetrical airfoil. (b) State and ...
Check whether the following impedance functions are LC, RL or RC? (a) Z(s) = ... amplitude 5 V. Find autocorrelation function and energy spectral density. [8+8].
3. Regeneration is possible in the fixed-bias transistor flip-flop if the base-to-base · voltage gain exceeds unity. Verify that this gain condition is satisfied provided ...
Set No - 2. (b) Write about bus structures. ... (b) Explain time-shared common bus in detail. [8+8] ... (c) Asynchronous serial transmission with one stop bits · [16].
Consider a telegraph source having two symbols, dot and dash. The dot duration · is 0.2s.The dash duration is 3 times the dot duration. the probability of the dots.
2. (a) Explain why hardening by quenching is followed by tempering. (b) Only mechanical properties not physical properties can be altered by heat · treatment.
Describe the methods of manipulating DNA for gene cloning purpose. [16] · 5. What are transposons? and describe about the types of bacterial transposons. [16].
Young's modulus = 35 Ã 1010 Pa and the specific surface energy = 2 J/m2 · (b) Give a brief account on the various stages of creep rate with reference to design.
(a) What is surface texture? Explain components of surface texture. (b) Explain Ball type plug gauge to measure big bore diameter. [8+8] · 8. A Rotameter has been calibrated in lit/min for water. It is to be used for metering · brine solution of spec
All Questions carry equal marks · ***** · 1. Explain the microbial production of aminoacids with one example. [16] · 2. What are biofuels? How these are produced ...
1. Explain tree based indexing and hash based indexing techniques. [16] · 2. Explain the .... 1. Explain in detail the structure of B+ tree with an example · [16].
(a) What is Radix-64 format? Explain how both PGP and S/MIME perform the · Radix-64 conversion is performed. (b) Describe the five principal services that ...
Farm in Maharashtra, supplied with water from a dug well pumping for 4 h a day, are given below. Determine the required discharge. [16]. 7. AjntuWorld.in ...
... carry equal marks · ***** · 1. List eight major functions of an exhaust nozzle. .... plane. Show the internal layout for swallowed air to reach the engine. Explain.
Enumerate difference between growth and non-growth associated product kinetics. [16] · 7. Write a brief note on biosensors, and describe how they are used for ...
