IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 COMPUTATIONAL MOLECULAR BIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. (a) UPGMA based upon Hamming distances is statistically consistent under the Jukes-Cantor model. Explain.
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(b) UPGMA based upon Jukes-Cantor distances is statistically consistent under the Jukes-Cantor model. Explain. [8+8] 2. Describe the different databases available for storage of protein resources.
[16]
3. Discuss the application of gene chips in various fields of Biotechnology.
[16]
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4. (a) What FASTA programs are available? What are they used for?
(b) What is an E-value? You do a databank search using FASTA with an amino acid sequence as query. The only reported match has an E value of 10. What does this mean for the similarity of two sequences. [8+8]
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5. Write short notes on:
(a) Substitution model in Phylogenetic analysis (b) CLUSTAL W. 6. Explain :
[8+8]
(a) BLAST similarity searching program.
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(b) What type of scoring matrix is used by BLAST? Explain about different substitution matrices? [8+8]
7. How can you analyze the physical properties of proteins.
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8. Discuss in detail how DNA is prapared? ?????
1
[16] [16]
Code No: 37182
R05
Set No - 2
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IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 COMPUTATIONAL MOLECULAR BIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
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1. The sequences shown below represent an optimal alignment of the first 50 nucleotides from the human and sheep preproinsulin genes. Estimate the number of substitutions that have occurred in this region. Since humans and sheep last shared a common ancestors using jukes cantor model. Human – ATGGCCCTGT GGATGCCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG SHEEP – ATGGCCCTGT GGACACGCCT GGTGCCCCTG CTGGCCCTGC TGGCACTCTG. [16]
or
2. Comment on the nature of information provided by structure classification of databases. [16] 3. How could you compare genomes with new template method?
[16]
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4. Who created BLAST and explain the type of scoring matrix used by BLAST? The role of BLAST and in database search tool in molecular biology? [16] 5. How well do threading method work?
[16]
6. (a) Describe how the BLOSUM scoring matrices were constructed. (b) Explain the principle of the Profiles method and its use in multiple sequence alignment. [8+8]
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7. Define the following terms: Expressed sequence tag(EST), positional cloning, Gene prediction, gene expression. Briefly explain each of them. [16] 8. Write short notes on the following : (a) Weighted parsimony
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(b) Unweighted Parsimony.
[8+8] ?????
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Code No: 37182
R05
Set No - 3
in
IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 COMPUTATIONAL MOLECULAR BIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ?????
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1. What are the chemical forces that stabilize native protein structures? What is the process by which a protein folds from an ensemble of denatured confirmations to a unique native state? [16] 2. What methods would you sue to visually estimate the number of clusters in a gene expression data. [16] 3. Explain:
or
(a) Which multiple-sequence alignment method should you use if you want to combine the output of several methods into one single alignment? (b) Which multiple-sequence alignment method should you use if you want to use the structural information associated with some of your sequences? [8+8]
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4. Discuss the properties and assumptions of the JukesCantor and the Kimura 2parameter models DNA evolution. [16] 5. Write short notes on:
(a) Small versus large parsimony
(b) Fitch and Sankoff algorithms.
6. What is protein engineering write about its advantages and disadvantates.
[8+8] [16]
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7. Write a note on:
(a) Pairwise database searching.
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(b) What are Heuristic alignment algorithms? Explain the best known heuristic algorithms BLAST & FASTA? [8+8]
8. Define EST. Describe methodology used the identification of genes. ?????
3
[16]
Code No: 37182
R05
Set No - 4
in
IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 COMPUTATIONAL MOLECULAR BIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. (a) Maximum likelihood (optimizing parameters under Jukes-Cantor) is statistically consistent under the GTR model. Explain.
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(b) Maximum likelihood (optimizing parameters under the K2P model) is statistically consistent under the Jukes-Cantor model. Explain. [8+8] 2. Distinguish between Distance, Parsimony and maximum likelihood approaches.[16] 3. What is BLAST outline the steps used by BLAST algorithm?
[16]
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4. What type of scoring matrix is used by FASTA? Explain about different substitution matrices? [16] 5. Name the two most important classification schemes coming under protein structure classification databases. Explain. [16] [16]
7. Describe the organization of nuclear DNA in eukaryotes.
[16]
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6. How the cloning is done? Explain the expression of genes?
8. Justify the statement-“Information of final structure of protein lies in its its primary structures. [16]
Explain. [8+8] · 2. Describe the different databases available for storage of protein resources. [16] ... acid sequence as query. The only reported match has an E ...
(b) Fretting corrosion. [8+8] · 4. Explain vapor phase inhibitors. [16] · 5. (a) Discuss various corrosion rate expressions with their merits and demerits. (b) Iron is ...
6. (a) Explain the various functions and tables associated with Ring station and · Source routing group of RMON token ring. (b) Explain the different alarms and ...
where you are studying. Write CSS rule such that there are three different para- · graph styles p1, p2 and p3. p1 style must use left and right margins of 20 pixels, ...
heit (oF),converts in to an absolute temperature in kelvins · T(oK) = 5/9[t(oF)-32.0]+273. [16] · 6. Sketch an inverse Nyquist plot for a system with g(s) =.
(b) How fast can the output of an Op-amp change by 10V, if its slew rate is · 1V/µs? .... (b) Explain the application of 555 timer as Linear ramp generator. [8+8].
How can you find virus concentration both directly and indirectly by particle counts · and measurement of infectious unit concentration. Define plaque forming ...
(b) Explain with a neat block diagram the operation of a continuously variable .... (a) Draw the block diagram of band pass binary data transmission system and.
(b) Describe the effect of lack of fusion in butt, fillet and lap welded joints. ... 8. (a) Describe the process of welding dissimilar metals by fusion welding technique?
[16] · 4. A chemical process is represented by the following set of equations : f1(x3,x4)=0 · ; f2(x5,x2) =0 ; f3(x6) = 0 ; f4(x6,x1) = 0 ; f5(x3,x2) = 0 & f6 (x4,x5,x1) =0.
A steel bolt of 650 sq.mm C.S.A passes centrally through a copper tube of .... 6. A wagon of weight 25 k N, attached to a wire rope, is moving at speed of 4 km.
Explain in detail how transposable elements could be used to clone the genes. [16] · 7. Write in detail about ISSR molecular markers and its applications. [16].
(a) With a neat sketch explain the generation of action potentials. (b) List out different bio electric potentials. (c) Explain how bio electric potentials are measured.
(b) Break-Even Point in Rupees,. (c) Margin of Safety. (d) It is proposed to reduce the selling price by 20%. What extra units should be sold to obtain the same ...
(b) Explain briefly about the threat's to data integrity and confidentiality. [8+8] · 5. Describe different clinical applications of computers in nuclear medicine. [16].
(b) Evaluate. â. â«. 0 dx. (1+x2)2 using residue theorem. [8+8]. 8. (a) Evaluate using Cauchy's Integral Formula â«. C. (z+1) dz. (z3â4z) where C is |z + 2| = 3. 2.
Code No: 35051. R05. Set No - 2. 6. (a) Obtain the state-space representation of the system given by the transfer func- · tion. (Conanical form of state space model) · Y (S) · U(S) · = T (S) = · 1 · (S+1)(S+2)(S+3) · (b) A system is characterized by
[16] · 4. What are channel,circuit and half circuit in the context of satellite telemetry sys- .... 3. Describe the conditions for total internal reflection within a fibre. [16].
(b) Describe a DC Servo Motor with a neat sketch. [8+8] · 4. (a) What is a timing belt? Discuss the use of Timing belts in power transmission? (b) Discuss various ...
(b) Consider any two networks of your choice which support Circuit switching or ... (b) Which is more efficient, circuit switching or packet switching? Why?
(a) What is the difference between logical I/O and device I/O? (b) Explain feed back scheduling policy. [8+8] · 2. (a) How can logical address space be ...
kerosene, and fuel oil must be limited as also shown in this table. There are no ... equipment is a function of x, the size (horsepower ) of the motor, namely.
