II B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009 MICROBIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Discuss the role of Lazarro Spallanzani and Ferdinand Cohn in disproving the doctrine of spontaneous generation of microorganisms. [16] [16]
3. Discuss general steps in viral replication.
[16]
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2. Explain why Whittaker’s Fivekingdom classification was disproved.
4. What is the relationship between retrovirus and oncogenes.
[16]
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5. Explain various media that are used for preservation of stock cultures. Discuss agar slants, sterile soil, spore preparations. [16]
6. What is culture media? Classify culture media based on function and composition. [16]
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7. How can you find virus concentration both directly and indirectly by particle counts and measurement of infectious unit concentration. Define plaque forming units, lethal dose and infectious dose. [16] 8. Describe phenols, alcohols and halogens in terms of their chemical nature, mechanism of action, mode of application, common uses and effectiveness and advantages and disadvantages. [16]
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Code No: 33028
R05
Set No - 2
1. Explain some common virus groups and their characteristics.
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II B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009 MICROBIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? [16]
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2. Write the effect of the following environmental factors on the growth of microorganisms. (a) Solutes. (b) PH (d) Osmotic pressure.
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(c) Oxygen
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3. What are pure cultures and why they are important? How are spread plate, streak plate and pour plates prepared. [16]
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4. As a microbiologist what is your concern about public health.
[16]
5. How does a plant +ve strand virus replicate and express its genome in plants. [16] 6. Describe the structure and chemistry of cell wall in Archaea.
[16]
7. How will you measure the killing rates of microorganisms? Explain some tests for measurement of killing rates. [16]
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8. Discuss the relationship between viruses and retrograde evolution.
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[16]
Code No: 33028
R05
Set No - 3
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II B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009 MICROBIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? [16]
2. Define assay. Explain various types of viral assays.
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1. Discuss the general characters of domain Bacteria, with suitable examples.
3. Write short notes on: (a) Tobacco Mosaic Virus (b) Poliovirus.
[8+8] [16]
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4. Explain the contributions of Louis Pasteur towards industrial microbiology.
5. What is pure culture? How do you obtain pure culture from mixed cultures? Explain three plating techniques for isolating individual cells. [16]
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6. What is radiation. Give the advantages and disadvantages of ultra violet light and ionizing radiation as sterilizing agents. Provide a few examples of how each is used for this purpose. [16] 7. Explain the reverse transcription process.
[16]
8. Define water activity and briefly discuss how it can be determined. Why is it diffcult for microorganisms to grow at low water activity (aw) values. [16]
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3
Code No: 33028
R05
Set No - 4
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II B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009 MICROBIOLOGY Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. What are nutrients? Describe major nutritional types of microorganisms.
[16]
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2. Discuss the discoveries of Redi, Needham, Spallanzani, and Ferdinand in disproving the theory of spontaneous generation. [16] 3. Discuss about the antimicrobial drugs and their uses.
[16]
4. How does a -ve strand virus replicate and express its genome.
[16]
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5. Write about the following: (a) Tissue culture cultivation of viruses. (b) Bacterial culture if viruses.
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6. Describe the structural properties of virus.
7. Briefly discuss about the three domains of life given by Woese etal.
[8+8] [16] [16]
8. What is numerical taxonomy and why are computers so important to this approach. [16]
How can you find virus concentration both directly and indirectly by particle counts · and measurement of infectious unit concentration. Define plaque forming ...
(b) Fretting corrosion. [8+8] · 4. Explain vapor phase inhibitors. [16] · 5. (a) Discuss various corrosion rate expressions with their merits and demerits. (b) Iron is ...
6. (a) Explain the various functions and tables associated with Ring station and · Source routing group of RMON token ring. (b) Explain the different alarms and ...
where you are studying. Write CSS rule such that there are three different para- · graph styles p1, p2 and p3. p1 style must use left and right margins of 20 pixels, ...
heit (oF),converts in to an absolute temperature in kelvins · T(oK) = 5/9[t(oF)-32.0]+273. [16] · 6. Sketch an inverse Nyquist plot for a system with g(s) =.
(b) How fast can the output of an Op-amp change by 10V, if its slew rate is · 1V/µs? .... (b) Explain the application of 555 timer as Linear ramp generator. [8+8].
(b) Explain with a neat block diagram the operation of a continuously variable .... (a) Draw the block diagram of band pass binary data transmission system and.
(b) Describe the effect of lack of fusion in butt, fillet and lap welded joints. ... 8. (a) Describe the process of welding dissimilar metals by fusion welding technique?
[16] · 4. A chemical process is represented by the following set of equations : f1(x3,x4)=0 · ; f2(x5,x2) =0 ; f3(x6) = 0 ; f4(x6,x1) = 0 ; f5(x3,x2) = 0 & f6 (x4,x5,x1) =0.
Explain. [8+8] · 2. Describe the different databases available for storage of protein resources. [16] ... acid sequence as query. The only reported match has an E ...
A steel bolt of 650 sq.mm C.S.A passes centrally through a copper tube of .... 6. A wagon of weight 25 k N, attached to a wire rope, is moving at speed of 4 km.
Explain in detail how transposable elements could be used to clone the genes. [16] · 7. Write in detail about ISSR molecular markers and its applications. [16].
(a) With a neat sketch explain the generation of action potentials. (b) List out different bio electric potentials. (c) Explain how bio electric potentials are measured.
(b) Break-Even Point in Rupees,. (c) Margin of Safety. (d) It is proposed to reduce the selling price by 20%. What extra units should be sold to obtain the same ...
(b) Explain briefly about the threat's to data integrity and confidentiality. [8+8] · 5. Describe different clinical applications of computers in nuclear medicine. [16].
(b) Evaluate. â. â«. 0 dx. (1+x2)2 using residue theorem. [8+8]. 8. (a) Evaluate using Cauchy's Integral Formula â«. C. (z+1) dz. (z3â4z) where C is |z + 2| = 3. 2.
Code No: 35051. R05. Set No - 2. 6. (a) Obtain the state-space representation of the system given by the transfer func- · tion. (Conanical form of state space model) · Y (S) · U(S) · = T (S) = · 1 · (S+1)(S+2)(S+3) · (b) A system is characterized by
[16] · 4. What are channel,circuit and half circuit in the context of satellite telemetry sys- .... 3. Describe the conditions for total internal reflection within a fibre. [16].
(b) Describe a DC Servo Motor with a neat sketch. [8+8] · 4. (a) What is a timing belt? Discuss the use of Timing belts in power transmission? (b) Discuss various ...
(b) Consider any two networks of your choice which support Circuit switching or ... (b) Which is more efficient, circuit switching or packet switching? Why?
(a) What is the difference between logical I/O and device I/O? (b) Explain feed back scheduling policy. [8+8] · 2. (a) How can logical address space be ...
kerosene, and fuel oil must be limited as also shown in this table. There are no ... equipment is a function of x, the size (horsepower ) of the motor, namely.
