IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOTECHNOLOGY FOR CROP IMPROVEMENT Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Write short notes on:
2. Discuss detailed protocol for isolated microspore culture. 3. Write short note on the following. (a) PCR in genetic engineering (b) Tagged gene for cloning.
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(c) High volume markers.
[16]
[5+6+5]
4. What do you understand by virus resistance. Discuss how antisense RNA is used to confer resistance to viruses. [16] 5. Write in detail about the economic aspects of transgenic crops which produce novel products. [16] 6. Explain in detail how transposable elements could be used to clone the genes. [16]
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7. Write in detail about ISSR molecular markers and its applications.
[16]
8. Write short notes on the following: (a) Threshold characters
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(b) Modifying genes
(c) Gene interaction
(d) Linkage.
[16] ?????
1
Code No: 37189/37190
R05
Set No - 2
in
IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOTECHNOLOGY FOR CROP IMPROVEMENT Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Describe how iron content in a plant or plant product can be elevated by introducing ferritin gene using transgenic method. [16]
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2. Illustrate the following (a) Gene trapping. (b) Enhancer trapping.
[16]
[16]
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3. What do you understand by DNA finger-printing. Explain its applications.
4. Describe in detail about the development of transgenic plant resistance to herbicide glyphosate and discuss its applications. [16]
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5. Describe how transgenic plants can be developed using mannitol dehydrogenase gene for salinity tolerance. [16] 6. What are the issues draw the attention of the plant breeding to use selective markers. [16] 7. Describe the prospects of plant breeding for crop improvement and describe how it has helped for improving productivity of staple crops in India, with suitable examples. [16]
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8. Can you expect double haploid production during anther culture in vitro. How you can differentiate between haploid and diploid plants during the growth until maturity. [16]
2
Code No: 37189/37190
R05
Set No - 3
in
IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOTECHNOLOGY FOR CROP IMPROVEMENT Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Can a whole plant be grown from a single cell, given the correct nutritional and hormonal environment?. [16]
3. Write short notes on the following: (a) Endoreduplication
(d) Gene amplification.
or
(b) Mitotic cross over (c) Transposable genetic elements
[16]
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2. List out the advantages and disadvantages of non targeted tagging.
[4×4]
4. Describe about sequence tagged micro satellite molecular markers and its uses. [16]
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5. Discuss the pedigree breeding in detail, explain its importance in breeding technology. [16] 6. What do you mean by insect resistance? Write in detail about the production of transgenic cotton for insect resistance using Bt gene. [16] 7. What are LEA proteins? Explain how LEA proteins can be used for conferring resistance to salt stressed water deficit. [16]
?????
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8. Why single - gene resistance are fragile and why not multiple gene resistance. [16]
3
Code No: 37189/37190
R05
Set No - 4
in
IV B.Tech I Semester Regular Examinations,Nov/Dec 2009 BIOTECHNOLOGY FOR CROP IMPROVEMENT Bio-Technology Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. (a) What is thermotherapy? How it is used for production of virus free plants.
[8+8]
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(b) How meristem tip culture is used for virus elimination?
2. Write in detail about the most specific uses of RFLP in crop improvement.
4. Explain in detail the technique of transposon tagging in genetic modification. [16] 5. Describe in detail the source and nature of Bt toxin gene and its use in transgenic plant production and developments in this area. [16] 6. Marker assisted selection has become a promising and potent approach for integrating biotechnology, comment. [16]
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7. Write in detail how glycerol -3-phosphate acetyl transferase gene is used for developing chilling tolerance. [16] 8. Explain the mechanism of inhibition of ethylene in transgenic plants using anti sense RNA genes during tomato ripening. [16]
Explain in detail how transposable elements could be used to clone the genes. [16] · 7. Write in detail about ISSR molecular markers and its applications. [16].
(b) Fretting corrosion. [8+8] · 4. Explain vapor phase inhibitors. [16] · 5. (a) Discuss various corrosion rate expressions with their merits and demerits. (b) Iron is ...
6. (a) Explain the various functions and tables associated with Ring station and · Source routing group of RMON token ring. (b) Explain the different alarms and ...
where you are studying. Write CSS rule such that there are three different para- · graph styles p1, p2 and p3. p1 style must use left and right margins of 20 pixels, ...
heit (oF),converts in to an absolute temperature in kelvins · T(oK) = 5/9[t(oF)-32.0]+273. [16] · 6. Sketch an inverse Nyquist plot for a system with g(s) =.
(b) How fast can the output of an Op-amp change by 10V, if its slew rate is · 1V/µs? .... (b) Explain the application of 555 timer as Linear ramp generator. [8+8].
How can you find virus concentration both directly and indirectly by particle counts · and measurement of infectious unit concentration. Define plaque forming ...
(b) Explain with a neat block diagram the operation of a continuously variable .... (a) Draw the block diagram of band pass binary data transmission system and.
(b) Describe the effect of lack of fusion in butt, fillet and lap welded joints. ... 8. (a) Describe the process of welding dissimilar metals by fusion welding technique?
[16] · 4. A chemical process is represented by the following set of equations : f1(x3,x4)=0 · ; f2(x5,x2) =0 ; f3(x6) = 0 ; f4(x6,x1) = 0 ; f5(x3,x2) = 0 & f6 (x4,x5,x1) =0.
Explain. [8+8] · 2. Describe the different databases available for storage of protein resources. [16] ... acid sequence as query. The only reported match has an E ...
A steel bolt of 650 sq.mm C.S.A passes centrally through a copper tube of .... 6. A wagon of weight 25 k N, attached to a wire rope, is moving at speed of 4 km.
(a) With a neat sketch explain the generation of action potentials. (b) List out different bio electric potentials. (c) Explain how bio electric potentials are measured.
(b) Break-Even Point in Rupees,. (c) Margin of Safety. (d) It is proposed to reduce the selling price by 20%. What extra units should be sold to obtain the same ...
(b) Explain briefly about the threat's to data integrity and confidentiality. [8+8] · 5. Describe different clinical applications of computers in nuclear medicine. [16].
(b) Evaluate. â. â«. 0 dx. (1+x2)2 using residue theorem. [8+8]. 8. (a) Evaluate using Cauchy's Integral Formula â«. C. (z+1) dz. (z3â4z) where C is |z + 2| = 3. 2.
Code No: 35051. R05. Set No - 2. 6. (a) Obtain the state-space representation of the system given by the transfer func- · tion. (Conanical form of state space model) · Y (S) · U(S) · = T (S) = · 1 · (S+1)(S+2)(S+3) · (b) A system is characterized by
[16] · 4. What are channel,circuit and half circuit in the context of satellite telemetry sys- .... 3. Describe the conditions for total internal reflection within a fibre. [16].
(b) Describe a DC Servo Motor with a neat sketch. [8+8] · 4. (a) What is a timing belt? Discuss the use of Timing belts in power transmission? (b) Discuss various ...
(b) Consider any two networks of your choice which support Circuit switching or ... (b) Which is more efficient, circuit switching or packet switching? Why?
(a) What is the difference between logical I/O and device I/O? (b) Explain feed back scheduling policy. [8+8] · 2. (a) How can logical address space be ...
kerosene, and fuel oil must be limited as also shown in this table. There are no ... equipment is a function of x, the size (horsepower ) of the motor, namely.
